- ￥940 - 2820
- 近岸蛋白(Novoprotein)
- 中国
- C10F
- 2025年12月11日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Recombinant Mouse Siglec-E (C-Fc)
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ￥940.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥2820.0 |
| 规格： | 500ug | 产品价格： | 询价 |
| 规格： | 1mg | 产品价格： | 询价 |
Recombinant Mouse Siglec-E (C-Fc) (C10F)
产品说明(Description)
Recombinant Mouse Sialic Acid Binding Ig-like Lectin E is produced by our Mammalian expression system and the target gene encoding Gln20-Phe355 is expressed with a Fc tag at the C-terminus.
Accession #: Q6PJ50
Known as: SiglecE; Siglec-E; Sialic Acid Binding Ig-like Lectin E
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Siglecs are sialic acid specific I-type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-set domain followed by varying numbers of Ig-like C2-set domains. Mouse Siglec-E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 - 85 kDa member of the CD33-related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec-9 is the most likely human ortholog of mouse Siglec-E. Siglec-E is expressed as a heavily N-glycosylated disulfide-linked homodimer and shows binding preference for disialic acids in the alpha 2-8 linkage. Siglec-E is up-regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec-E signaling negatively regulates the LPS-induced production of TNF- alpha and IL-6 by macrophages. Its up-regulation in macrophages parallels the development of endotoxin tolerance. Siglec-E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL-12 p40 production.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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