Recombinant Cynomolgus Neuropi

Recombinant Cynomolgus Neuropilin-1 (C-6His) (C11S)

产品说明(Description)

Recombinant Cynomolgus Neuropilin-1 is produced by our Mammalian expression system and the target gene encoding Phe22-Gly613 is expressed with a 6His tag at the C-terminus.

Accession #: G7PEQ1

Known as: CD304; NRP1; NRPNP1; VEGF165R; BDCA4; Neuropilin-1

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. Neuropilin-1 binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY