N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media.

N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate.

N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and EGF or used with DMEM.

Learn more about N-2 Supplement formulation.

Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.

For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.

Components	Molecular Weight	Concentration (mg/L)	mM
Proteins
Human Transferrin (Holo)	10000	10000	1
Insulin Recombinant Full Chain	5807.7	500	0.0861
Other Components
Progesterone	314.47	0.63	0.002
Putrescine	161	1611	10.01
Selenite	173	0.52	0.00301