- ￥1089.79
- MP Biomedicals
- 0215000191
- 2026年01月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
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- CAS号：
7240-90-6
- 英文名：
5-Bromo-4-Chloro-3-Indolyl-Β-D-Galactopyranoside
- 供应商：
MP Biomedicals
- 规格：
0215000191(1g)/0215000105(5 MG)/0215000110(10 MG)/0215000150(50 MG)/0215000180(100mg)/0215000183(250 MG)/0215000190(500 MG)
| 规格： | 0215000191(1g) | 产品价格： | ￥1089.79 |
|---|---|---|---|
| 规格： | 0215000105(5 MG) | 产品价格： | 询价 |
| 规格： | 0215000110(10 MG) | 产品价格： | 询价 |
| 规格： | 0215000150(50 MG) | 产品价格： | 询价 |
| 规格： | 0215000180(100mg) | 产品价格： | 询价 |
| 规格： | 0215000183(250 MG) | 产品价格： | 询价 |
| 规格： | 0215000190(500 MG) | 产品价格： | 询价 |
5-溴-4-氯-3-吲哚-β-D-半乳糖苷，通常称之为半乳糖苷，是 b-牛乳糖的组织化学基质。半乳糖苷由 b-牛乳糖裂解产生的一种不溶性蓝色沉淀。半乳糖苷主要用于分子生物学以检测b-牛乳糖的活性，更常用的是作为报告基因。DNA插入到虫胶Z区可导致b-牛乳糖失活。由互补作用得到的虫胶-细菌菌落外观是蓝色的，然而含有质粒的细菌菌落将DNA插入到虫胶Z区，将不能互补并出现白色。
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside is used as indigogenic substrate for b - galactosidase, for detection of b - galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. X-Gal is a chromogenic substrate for ß-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
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文献和实验, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
Materials Fixative 2% Paraformaldehyde (in PBS) Staining solution 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, 2 mM MgCI, 0.01% sodium deoxycholate 0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock 40 mg
Detection of -Galactosidase Activity: X-gal Staining
X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked
