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文献和实验Gene Knockdown Analysis by Double-Stranded RNA Injection
The discovery of RNAi, in which double-stranded RNA (dsRNA) suppresses the translation of homologous mRNA, has had a huge impact on evolutionary genetics by enabling the analysis of loss-of-function phenotypes in organisms in which classical
Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans
The nematode Caenorhabditis elegans is often employed in investigations of diverse aspects of biology, including behavior, development, basic cellular processes, and disease states. The ability to utilize double-stranded RNA (dsRNA
Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA
Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals
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