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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 英文名:
Ethyl Violet Aziode Broth
- 保质期:
三年
- 供应商:
上海晅科生物科技有限公司
- 保存条件:
2-8℃
- 规格:
250g-1kg
Ethyl Violet Aziode Broth
英文名称:Ethyl Violet Aziode Broth
用于链球菌的增菌培养
成分(g/L)
| 酪蛋白胨 | 13.5 |
| 酵母浸粉 | 6.5 |
| 葡萄糖 | 5.0 |
| 氯化钠 | 5.0 |
| 磷酸氢二钠 | 2.7 |
| 磷酸二氢钠 | 2.7 |
| 叠dan钠 | 0.4 |
| 乙基紫 | 0.00083 |
| pH值7.0±0.2 | 25℃ |
用法
称取本品 35.8g,溶解于1000ml 蒸馏水中,分装,121℃高压灭菌 15 分钟,备用。
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| 含225ml磷酸盐缓冲液均质袋 | Phosphate Buffered Saline | |
| 含90ml磷酸盐缓冲液均质袋 | ||
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| 含225ml mEC+n肉汤均质袋 | mEC+n Broth | |
| 新生霉素 | Novobiocin | |
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| 萘啶酮酸(5.0mg) | ||
| 吖啶黄素(3.0mg) | Acridine Flavin | |
| 含225ml FB1增菌液均质袋 | FB1 Broth |
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文献和实验.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical
30-50 μm fixed frozen/vibratome sections, mounted on slides Beakers to mix and hold solutions Incubation chamber Solutions Cresyl fast violet Glacial acetic acid 100% ethyl alcohol Chloroform 95% ethyl alcohol / 5% deionized H2
Butanol, water saturated Cadmium Chloride Stock Solution Calcium Chloride for Competent Cells Calcium Chloride Stock Solution CIP Buffer (10x) Coomassie Destain Coomassie Stain Crystal Violet Stock Solution
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