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参照说明书
- 英文名：
Dde Azide Agarose
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
2 ml
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A or protein G)(2) Ice-cold Tris-buffered saline (TBS). See recipe below(3) 5% sodium azide solution
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thing, like plasmid, gonome DNA can be broken. RNA is vunebable, like primers,siRNA; protein is easily denatured and degraded so always on ice, to antibody and milk we need add some azide aginst bacteria. 3.we should know how to get the imformation
RUVKUN Antibody Staining Protocol revised 10/29/90
1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES: 50 mM Tris Cl pH 9.5 5 mM MgCl 2% agarose -dissolve 2 mg n-propyl gallate in 70
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