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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated
- 规格:
0.5mg
| Host Goat |
|
Clonality Polyclonal
|
|
Purity Affinity purified IgG, F(ab)'2 fragment
|
|
Format Lyophilized
|
|
Quantity 0.5 mg
|
|
Reconstitution For reconstitution add 0.55 ml of sterile water
|
|
Storage Store lyophilized material at 2-8°C.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
|
名称:Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated
货号:AS10 1039
品牌:Agrisera
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
这类抗体只与 IgG 重链反应,通常不能和 IgA 或 IgM 任何表位反应。 注意:不是所有 anti-IgG, Fc 抗体和所有亚型的 IgG 反应能力一致。要找和四种 IgG 亚类反应能力差不多的 抗鼠IgG, Fc γ抗体的话,应当选羊抗鼠IgG( 亚类1+2a+2b+3), Fc γ特异性片段(min X Hu,Bov,Rb Sr Prot) 。 Anti-IgG , F(ab ’)2 fragment specific 这类抗体和 IgG 的 F(ab
-IgG (H+L)二抗与IgG的重链和轻链都有结合反应,即与IgG的Fc的F(ab’)2片段都能结合;由于IgG、IgM、IgA都有保守的轻链结构域,因此Anti-IgG (H+L)与它们都有交叉反应。而Anti-IgG, F(ab’)2 fragment经过了IgG Fc片段的吸附,因此只与IgG的F(ab’)2片段结合,然而IgG、IgM、IgA都有保守的轻链结构域,因此与它们也有交叉反应。 【Anti-IgG(H+L)、Anti-IgG (gamma)、Anti-IgM (mu
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