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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated
- 规格:
0.5mg
| Host Goat |
|
Clonality Polyclonal
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|
Purity Affinity purified IgG, F(ab)'2 fragment
|
|
Format Lyophilized
|
|
Quantity 0.5 mg
|
|
Reconstitution For reconstitution add 0.55 ml of sterile water
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Storage Store lyophilized material at 2-8°C.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
|
名称:Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated
货号:AS10 1039
品牌:Agrisera
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
)→ IgG F(ab′)2 pFc ′ 意义 阐明Ig分子生物学作用; 构建基因工程抗体。 Ig 分子上的两条重链和两条轻链是由二硫键连接的,因此它可以被巯基试剂还原成单链或双链;在Ig 分子的铰链区,由于富含脯氨酸而易于称为蛋白酶的酶切位点。 (1)木瓜蛋白酶水解片段 木瓜蛋白酶(papain )水解IgG 的部位是在铰链区二硫键连接的2 条重链的近N 端,裂解后可得到三个片段,其中两个片段相同:2 个相同片段称为抗原结合片段(fragment
作者:陈英剑 李芳秋 摘要 以rhCyPA为包被抗原,用间接ELISA检测了45例SLE病人血浆抗CyPA自身抗体的IgG、IgA、IgM三种类型,并探讨了此抗体与多项临床指标的相关性。结果显示,IgG、IgA、IgM三类抗CyPA自身抗体的阳性率分别为36%,20%,18%,总阳性率为48%。存在皮肤粘膜损害、白细胞减少、发热、并联能表现或活动期的SLE病人抗CyPA自身抗体阳性率明显高于无相应症状或非活动期SLE病人。 An investigation
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