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- 英文名:
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- 供应商:
无锡云萃生物
- CAS号:
68649-12-7
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Concentrating poly(A)+ mRNA Source: Protocol Online Abstract: Simple precipitation method of concentrating poly(A)+ mRNA
to generate sequence-tagged sites (STSs) (11) from cDNAs (12,13). Protocol 1. Synthesize anchored poly (dT)17 with anchors of (dA) or (dC) or (dG) at the 3' end on a DNA synthesizer and use after purification on Oligonucleotide Purification Cartridges
of total RNA) into a microfuge tube. Spin for 3 min at 12,000 rpm. Discard the supernatant. Gently suspend the latex in the same volume of 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.1% SDS. Spin again. Gently suspend the latex in the same buffer. 1. Add
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