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- 文献和实验
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- 英文名:
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- 供应商:
无锡云萃生物
- CAS号:
99891-77-7
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Crystallization of Kinesin Family Motor Proteins
parameters.Crystals of monomeric rat Kinesin-1Table 1: Crystallization conditions for kinesin motor protein constructsConstructMethodConditionsReferencesHuman Kinesin-1 hK349Sitting drop 4°C5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v
Protein extraction from whole tissues for IEF
volume of 100 mM Tris pH 8.8 stir overnight repeat remove most of the Tris leaving a cm layer to cover the phenol your need 2.5 ml of phenol for 1 g of tissue 0.1 M ammonium acetate in 100% methanol (100 ml) - store at -20 C 0.77 g/100 ml
0.25M HCl (1L) 20.66mL HCl 979.34mL dH2 O *add acid to water* 1M ammonium acetate, 0.02M NaOH (1L) 77.08g ammonium acetate 0.8g NaOH Fill with dH2 O 0.5X SSC, 0.1% SDS (1L) 25mL 20
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