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- 文献和实验
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- 供应商:
无锡云萃生物
- CAS号:
79114-77-5
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5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Crystallization of Kinesin Family Motor Proteins
.,2001; Nitta et al.,2004); the Kinesin-5 (formerly BimC)motor,monomeric Eg5 (Turner et al.,2001); and the Kinesin-13 (formerly MCAK)motor,PfKinI (Shipley et al.,2004).The following tables summarize the crystallization conditions and some of the crystal
Protein extraction from whole tissues for IEF
volume of 100 mM Tris pH 8.8 stir overnight repeat remove most of the Tris leaving a cm layer to cover the phenol your need 2.5 ml of phenol for 1 g of tissue 0.1 M ammonium acetate in 100% methanol (100 ml) - store at -20 C 0.77 g/100 ml
0.25M HCl (1L) 20.66mL HCl 979.34mL dH2 O *add acid to water* 1M ammonium acetate, 0.02M NaOH (1L) 77.08g ammonium acetate 0.8g NaOH Fill with dH2 O 0.5X SSC, 0.1% SDS (1L) 25mL 20
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