pAd-PL-DEST腺病毒质粒

基本信息
名称：pAd-PL-DEST腺病毒质粒
别称: pAd/PL-DEST

复制子:	pUC
原核抗性:	Amp
克隆菌株:	DB3.1
培养条件:	37度

质粒属性

载体宿主：	哺乳细胞,腺病毒
载体用途：	蛋白表达
基因种属：
基因类型：	GateWay
原核抗性：	Amp
真核抗性：
荧光蛋白：

质粒简介
  pAd-PL-DEST pAd/PL-DEST是一个腺病毒Ad系列Gateway系统质粒。

质粒图谱


质粒序列
LOCUS       Exported               34864 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..34864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     protein_bind    512..636
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        661..691
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             745..1404
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1746..2051
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLL-SDK
                     VSRELYPVVHIGDESWRMMTTD-MASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2092..2216)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5766..5869
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(12964..12975)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      20448..20457
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     repeat_region   32680..32782
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(33006..33594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(33746..34606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPA?MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(34607..34711)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagtcgaag cttggatccg gtacctctag
      481 aattctcgag cggccgctag cgacatcgat cacaagtttg tacaaaaaag ctgaacgaga
      541 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      601 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
      661 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga
      721 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      781 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      841 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      901 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      961 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1021 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1081 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1141 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1201 ttcaccagtt ttgatttaaa cgtggccaat atggacaatt tcttcgcccc cgttttcacc
     1261 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1321 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1381 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaaac cagataacag
     1441 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     1501 agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca
     1561 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     1621 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     1681 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     1741 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1801 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     1861 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     1921 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     1981 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2041 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     2101 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     2161 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatc
     2221 gattcgacag atcactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg
     2281 tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa
     2341 ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg
     2401 ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc
     2461 tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc
     2521 cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc
     2581 gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt
     2641 ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct
     2701 gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa
     2761 taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc tttatttagg
     2821 ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat
     2881 tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca taagcccgtc
     2941 tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt tgtagatgat
     3001 ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta gcaagctgat
     3061 tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat
     3121 acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt tcccagccat
     3181 atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg tgcacttggg
     3241 aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc
     3301 tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg cggcggcctg
     3361 ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga gatcgtcata
     3421 ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg ttccatccgg
     3481 cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt cagatggggg
     3541 gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg agatcagctg
     3601 ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac
     3661 acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat ccctgagcag
     3721 gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca aatccgccag
     3781 aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt
     3841 gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca ggcggtccca
     3901 cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt tcgcgggttg
     3961 gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct
     4021 ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg
     4081 ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg
     4141 tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc cagcccctcc
     4201 gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga ggggcagtgc
     4261 agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga gtaggcatcc
     4321 gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc tggccgttcg
     4381 gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct ggtttccatg
     4441 agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga
     4501 ggcctgtcct cgagcggtgt tccgcggtcc tcctcgtata gaaactcgga ccactctgag
     4561 acaaaggctc gcgtccaggc cagcacgaag gaggctaagt gggaggggta gcggtcgttg
     4621 tccactaggg ggtccactcg ctccagggtg tgaagacaca tgtcgccctc ttcggcatca
     4681 aggaaggtga ttggtttgta ggtgtaggcc acgtgaccgg gtgttcctga aggggggcta
     4741 taaaaggggg tgggggcgcg ttcgtcctca ctctcttccg catcgctgtc tgcgagggcc
     4801 agctgttggg gtgagtactc cctctgaaaa gcgggcatga cttctgcgct aagattgtca
     4861 gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg
     4921 gccgcatcca tctggtcaga aaagacaatc tttttgttgt caagcttggt ggcaaacgac
     4981 ccgtagaggg cgttggacag caacttggcg atggagcgca gggtttggtt tttgtcgcga
     5041 tcggcgcgct ccttggccgc gatgtttagc tgcacgtatt cgcgcgcaac gcaccgccat
     5101 tcgggaaaga cggtggtgcg ctcgtcgggc accaggtgca cgcgccaacc gcggttgtgc
     5161 agggtgacaa ggtcaacgct ggtggctacc tctccgcgta ggcgctcgtt ggtccagcag
     5221 aggcggccgc ccttgcgcga gcagaatggc ggtagggggt ctagctgcgt ctcgtccggg
     5281 gggtctgcgt ccacggtaaa gaccccgggc agcaggcgcg cgtcgaagta gtctatcttg
     5341 catccttgca agtctagcgc ctgctgccat gcgcgggcgg caagcgcgcg ctcgtatggg
     5401 ttgagtgggg gaccccatgg catggggtgg gtgagcgcgg aggcgtacat gccgcaaatg
     5461 tcgtaaacgt agaggggctc tctgagtatt ccaagatatg tagggtagca tcttccaccg
     5521 cggatgctgg cgcgcacgta atcgtatagt tcgtgcgagg gagcgaggag gtcgggaccg
     5581 aggttgctac gggcgggctg ctctgctcgg aagactatct gcctgaagat ggcatgtgag
     5641 ttggatgata tggttggacg ctggaagacg ttgaagctgg cgtctgtgag acctaccgcg
     5701 tcacgcacga aggaggcgta ggagtcgcgc agcttgttga ccagctcggc ggtgacctgc
     5761 acgtctaggg cgcagtagtc cagggtttcc ttgatgatgt catacttatc ctgtcccttt
     5821 tttttccaca gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc
     5881 ggaaacccgt cggcctccga acggtaagag cctagcatgt agaactggtt gacggcctgg
     5941 taggcgcagc atcccttttc tacgggtagc gcgtatgcct gcgcggcctt ccggagcgag
     6001 gtgtgggtga gcgcaaaggt gtccctgacc atgactttga ggtactggta tttgaagtca
     6061 gtgtcgtcgc atccgccctg ctcccagagc aaaaagtccg tgcgcttttt ggaacgcgga
     6121 tttggcaggg cgaaggtgac atcgttgaag agtatctttc ccgcgcgagg cataaagttg
     6181 cgtgtgatgc ggaagggtcc cggcacctcg gaacggttgt taattacctg ggcggcgagc
     6241 acgatctcgt caaagccgtt gatgttgtgg cccacaatgt aaagttccaa gaagcgcggg
     6301 atgcccttga tggaaggcaa ttttttaagt tcctcgtagg tgagctcttc aggggagctg
     6361 agcccgtgct ctgaaagggc ccagtctgca agatgagggt tggaagcgac gaatgagctc
     6421 cacaggtcac gggccattag catttgcagg tggtcgcgaa aggtcctaaa ctggcgacct
     6481 atggccattt tttctggggt gatgcagtag aaggtaagcg ggtcttgttc ccagcggtcc
     6541 catccaaggt tcgcggctag gtctcgcgcg gcagtcacta gaggctcatc tccgccgaac
     6601 ttcatgacca gcatgaaggg cacgagctgc ttcccaaagg cccccatcca agtataggtc
     6661 tctacatcgt aggtgacaaa gagacgctcg gtgcgaggat gcgagccgat cgggaagaac
     6721 tggatctccc gccaccaatt ggaggagtgg ctattgatgt ggtgaaagta gaagtccctg
     6781 cgacgggccg aacactcgtg ctggcttttg taaaaacgtg cgcagtactg gcagcggtgc
     6841 acgggctgta catcctgcac gaggttgacc tgacgaccgc gcacaaggaa gcagagtggg
     6901 aatttgagcc cctcgcctgg cgggtttggc tggtggtctt ctacttcggc tgcttgtcct
     6961 tgaccgtctg gctgctcgag gggagttacg gtggatcgga ccaccacgcc gcgcgagccc
     7021 aaagtccaga tgtccgcgcg cggcggtcgg agcttgatga caacatcgcg cagatgggag
     7081 ctgtccatgg tctggagctc ccgcggcgtc aggtcaggcg ggagctcctg caggtttacc
     7141 tcgcatagac gggtcagggc gcgggctaga tccaggtgat acctaatttc caggggctgg
     7201 ttggtggcgg cgtcgatggc ttgcaagagg ccgcatcccc gcggcgcgac tacggtaccg
     7261 cgcggcgggc ggtgggccgc gggggtgtcc ttggatgatg catctaaaag cggtgacgcg
     7321 ggcgagcccc cggaggtagg gggggctccg gacccgccgg gagagggggc aggggcacgt
     7381 cggcgccgcg cgcgggcagg agctggtgct gcgcgcgtag gttgctggcg aacgcgacga
     7441 cgcggcggtt gatctcctga atctggcgcc tctgcgtgaa gacgacgggc ccggtgagct
     7501 tgagcctgaa agagagttcg acagaatcaa tttcggtgtc gttgacggcg gcctggcgca
     7561 aaatctcctg cacgtctcct gagttgtctt gataggcgat ctcggccatg aactgctcga
     7621 tctcttcctc ctggagatct ccgcgtccgg ctcgctccac ggtggcggcg aggtcgttgg
     7681 aaatgcgggc catgagctgc gagaaggcgt tgaggcctcc ctcgttccag acgcggctgt
     7741 agaccacgcc cccttcggca tcgcgggcgc gcatgaccac ctgcgcgaga ttgagctcca
     7801 cgtgccgggc gaagacggcg tagtttcgca ggcgctgaaa gaggtagttg agggtggtgg
     7861 cggtgtgttc tgccacgaag aagtacataa cccagcgtcg caacgtggat tcgttgatat
     7921 cccccaaggc ctcaaggcgc tccatggcct cgtagaagtc cacggcgaag ttgaaaaact
     7981 gggagttgcg cgccgacacg gttaactcct cctccagaag acggatgagc tcggcgacag
     8041 tgtcgcgcac ctcgcgctca aaggctacag gggcctcttc ttcttcttca atctcctctt
     8101 ccataagggc ctccccttct tcttcttctg gcggcggtgg gggagggggg acacggcggc
     8161 gacgacggcg caccgggagg cggtcgacaa agcgctcgat catctccccg cggcgacggc
     8221 gcatggtctc ggtgacggcg cggccgttct cgcgggggcg cagttggaag acgccgcccg
     8281 tcatgtcccg gttatgggtt ggcggggggc tgccatgcgg cagggatacg gcgctaacga
     8341 tgcatctcaa caattgttgt gtaggtactc cgccgccgag ggacctgagc gagtccgcat
     8401 cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc
     8461 tgagcaccgt ggcgggcggc agcgggcggc ggtcggggtt gtttctggcg gaggtgctgc
     8521 tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgacaga agcaccatgt
     8581 ccttgggtcc ggcctgctga atgcgcaggc ggtcggccat gccccaggct tcgttttgac
     8641 atcggcgcag gtctttgtag tagtcttgca tgagcctttc taccggcact tcttcttctc
     8701 cttcctcttg tcctgcatct cttgcatcta tcgctgcggc ggcggcggag tttggccgta
     8761 ggtggcgccc tcttcctccc atgcgtgtga ccccgaagcc cctcatcggc tgaagcaggg
     8821 ctaggtcggc gacaacgcgc tcggctaata tggcctgctg cacctgcgtg agggtagact
     8881 ggaagtcatc catgtccaca aagcggtggt atgcgcccgt gttgatggtg taagtgcagt
     8941 tggccataac ggaccagtta acggtctggt gacccggctg cgagagctcg gtgtacctga
     9001 gacgcgagta agccctcgag tcaaatacgt agtcgttgca agtccgcacc aggtactggt
     9061 atcccaccaa aaagtgcggc ggcggctggc ggtagagggg ccagcgtagg gtggccgggg
     9121 ctccgggggc gagatcttcc aacataaggc gatgatatcc gtagatgtac ctggacatcc
     9181 aggtgatgcc ggcggcggtg gtggaggcgc gcggaaagtc gcggacgcgg ttccagatgt
     9241 tgcgcagcgg caaaaagtgc tccatggtcg ggacgctctg gccggtcagg cgcgcgcaat
     9301 cgttgacgct ctagaccgtg caaaaggaga gcctgtaagc gggcactctt ccgtggtctg
     9361 gtggataaat tcgcaagggt atcatggcgg acgaccgggg ttcgagcccc gtatccggcc
     9421 gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac
     9481 aacgggggag tgctcctttt ggcttccttc caggcgcggc ggctgctgcg ctagcttttt
     9541 tggccactgg ccgcgcgcag cgtaagcggt taggctggaa agcgaaagca ttaagtggct
     9601 cgctccctgt agccggaggg ttattttcca agggttgagt cgcgggaccc ccggttcgag
     9661 tctcggaccg gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc
     9721 ttgcaaattc ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg
     9781 tgctgcggca gatgcgcccc cctcctcagc agcggcaaga gcaagagcag cggcagacat
     9841 gcagggcacc ctcccctcct cctaccgcgt caggaggggc gacatccgcg gttgacgcgg
     9901 cagcagatgg tgattacgaa cccccgcggc gccgggcccg gcactacctg gacttggagg
     9961 agggcgaggg cctggcgcgg ctaggagcgc cctctcctga gcggtaccca agggtgcagc
    10021 tgaagcgtga tacgcgtgag gcgtacgtgc cgcggcagaa cctgtttcgc gaccgcgagg
    10081 gagaggagcc cgaggagatg cgggatcgaa agttccacgc agggcgcgag ctgcggcatg
    10141 gcctgaatcg cgagcggttg ctgcgcgagg aggactttga gcccgacgcg cgaaccggga
    10201 ttagtcccgc gcgcgcacac gtggcggccg ccgacctggt aaccgcatac gagcagacgg
    10261 tgaaccagga gattaacttt caaaaaagct ttaacaacca cgtgcgtacg cttgtggcgc
    10321 gcgaggaggt ggctatagga ctgatgcatc tgtgggactt tgtaagcgcg ctggagcaaa
    10381 acccaaatag caagccgctc atggcgcagc tgttccttat agtgcagcac agcagggaca
    10441 acgaggcatt cagggatgcg ctgctaaaca tagtagagcc cgagggccgc tggctgctcg
    10501 atttgataaa catcctgcag agcatagtgg tgcaggagcg cagcttgagc ctggctgaca
    10561 aggtggccgc catcaactat tccatgctta gcctgggcaa gttttacgcc cgcaagatat
    10621 accatacccc ttacgttccc atagacaagg aggtaaagat cgaggggttc tacatgcgca
    10681 tggcgctgaa ggtgcttacc ttgagcgacg acctgggcgt ttatcgcaac gagcgcatcc
    10741 acaaggccgt gagcgtgagc cggcggcgcg agctcagcga ccgcgagctg atgcacagcc
    10801 tgcaaagggc cctggctggc acgggcagcg gcgatagaga ggccgagtcc tactttgacg
    10861 cgggcgctga cctgcgctgg gccccaagcc gacgcgccct ggaggcagct ggggccggac
    10921 ctgggctggc ggtggcaccc gcgcgcgctg gcaacgtcgg cggcgtggag gaatatgacg
    10981 aggacgatga gtacgagcca gaggacggcg agtactaagc ggtgatgttt ctgatcagat
    11041 gatgcaagac gcaacggacc cggcggtgcg ggcggcgctg cagagccagc cgtccggcct
    11101 taactccacg gacgactggc gccaggtcat ggaccgcatc atgtcgctga ctgcgcgcaa
    11161 tcctgacgcg ttccggcagc agccgcaggc caaccggctc tccgcaattc tggaagcggt
    11221 ggtcccggcg cgcgcaaacc ccacgcacga gaaggtgctg gcgatcgtaa acgcgctggc
    11281 cgaaaacagg gccatccggc ccgacgaggc cggcctggtc tacgacgcgc tgcttcagcg
    11341 cgtggctcgt tacaacagcg gcaacgtgca gaccaacctg gaccggctgg tgggggatgt
    11401 gcgcgaggcc gtggcgcagc gtgagcgcgc gcagcagcag ggcaacctgg gctccatggt
    11461 tgcactaaac gccttcctga gtacacagcc cgccaacgtg ccgcggggac aggaggacta
    11521 caccaacttt gtgagcgcac tgcggctaat ggtgactgag acaccgcaaa gtgaggtgta
    11581 ccagtctggg ccagactatt ttttccagac cagtagacaa ggcctgcaga ccgtaaacct
    11641 gagccaggct ttcaaaaact tgcaggggct gtggggggtg cgggctccca caggcgaccg
    11701 cgcgaccgtg tctagcttgc tgacgcccaa ctcgcgcctg ttgctgctgc taatagcgcc
    11761 cttcacggac agtggcagcg tgtcccggga cacataccta ggtcacttgc tgacactgta
    11821 ccgcgaggcc ataggtcagg cgcatgtgga cgagcatact ttccaggaga ttacaagtgt
    11881 cagccgcgcg ctggggcagg aggacacggg cagcctggag gcaaccctaa actacctgct
    11941 gaccaaccgg cggcagaaga tcccctcgtt gcacagttta aacagcgagg aggagcgcat
    12001 tttgcgctac gtgcagcaga gcgtgagcct taacctgatg cgcgacgggg taacgcccag
    12061 cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc atgtatgcct caaaccggcc
    12121 gtttatcaac cgcctaatgg actacttgca tcgcgcggcc gccgtgaacc ccgagtattt
    12181 caccaatgcc atcttgaacc cgcactggct accgccccct ggtttctaca ccgggggatt
    12241 cgaggtgccc gagggtaacg atggattcct ctgggacgac atagacgaca gcgtgttttc
    12301 cccgcaaccg cagaccctgc tagagttgca acagcgcgag caggcagagg cggcgctgcg
    12361 aaaggaaagc ttccgcaggc caagcagctt gtccgatcta ggcgctgcgg ccccgcggtc
    12421 agatgctagt agcccatttc caagcttgat agggtctctt accagcactc gcaccacccg
    12481 cccgcgcctg ctgggcgagg aggagtacct aaacaactcg ctgctgcagc cgcagcgcga
    12541 aaaaaacctg cctccggcat ttcccaacaa cgggatagag agcctagtgg acaagatgag
    12601 tagatggaag acgtacgcgc aggagcacag ggacgtgcca ggcccgcgcc cgcccacccg
    12661 tcgtcaaagg cacgaccgtc agcggggtct ggtgtgggag gacgatgact cggcagacga
    12721 cagcagcgtc ctggatttgg gagggagtgg caacccgttt gcgcaccttc gccccaggct
    12781 ggggagaatg ttttaaaaaa aaaaaagcat gatgcaaaat aaaaaactca ccaaggccat
    12841 ggcaccgagc gttggttttc ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag
    12901 gaaggtcctc ctccctccta cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg
    12961 ggttctccct tcgatgctcc cctggacccg ccgtttgtgc ctccgcggta cctgcggcct
    13021 accgggggga gaaacagcat ccgttactct gagttggcac ccctattcga caccacccgt
    13081 gtgtacctgg tggacaacaa gtcaacggat gtggcatccc tgaactacca gaacgaccac
    13141 agcaactttc tgaccacggt cattcaaaac aatgactaca gcccggggga ggcaagcaca
    13201 cagaccatca atcttgacga ccggtcgcac tggggcggcg acctgaaaac catcctgcat
    13261 accaacatgc caaatgtgaa cgagttcatg tttaccaata agtttaaggc gcgggtgatg
    13321 gtgtcgcgct tgcctactaa ggacaatcag gtggagctga aatacgagtg ggtggagttc
    13381 acgctgcccg agggcaacta ctccgagacc atgaccatag accttatgaa caacgcgatc
    13441 gtggagcact acttgaaagt gggcagacag aacggggttc tggaaagcga catcggggta
    13501 aagtttgaca cccgcaactt cagactgggg tttgaccccg tcactggtct tgtcatgcct
    13561 ggggtatata caaacgaagc cttccatcca gacatcattt tgctgccagg atgcggggtg
    13621 gacttcaccc acagccgcct gagcaacttg ttgggcatcc gcaagcggca acccttccag
    13681 gagggcttta ggatcaccta cgatgatctg gagggtggta acattcccgc actgttggat
    13741 gtggacgcct accaggcgag cttgaaagat gacaccgaac agggcggggg tggcgcaggc
    13801 ggcagcaaca gcagtggcag cggcgcggaa gagaactcca acgcggcagc cgcggcaatg
    13861 cagccggtgg aggacatgaa cgatcatgcc attcgcggcg acacctttgc cacacgggct
    13921 gaggagaagc gcgctgaggc cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc
    13981 gaggtcgaga agcctcagaa gaaaccggtg atcaaacccc tgacagagga cagcaagaaa
    14041 cgcagttaca acctaataag caatgacagc accttcaccc agtaccgcag ctggtacctt
    14101 gcatacaact acggcgaccc tcagaccgga atccgctcat ggaccctgct ttgcactcct
    14161 gacgtaacct gcggctcgga gcaggtctac tggtcgttgc cagacatgat gcaagacccc
    14221 gtgaccttcc gctccacgcg ccagatcagc aactttccgg tggtgggcgc cgagctgttg
    14281 cccgtgcact ccaagagctt ctacaacgac caggccgtct actcccaact catccgccag
    14341 tttacctctc tgacccacgt gttcaatcgc tttcccgaga accagatttt ggcgcgcccg
    14401 ccagccccca ccatcaccac cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg
    14461 ctaccgctgc gcaacagcat cggaggagtc cagcgagtga ccattactga cgccagacgc
    14521 cgcacctgcc cctacgttta caaggccctg ggcatagtct cgccgcgcgt cctatcgagc
    14581 cgcacttttt gagcaagcat gtccatcctt atatcgccca gcaataacac aggctggggc
    14641 ctgcgcttcc caagcaagat gtttggcggg gccaagaagc gctccgacca acacccagtg
    14701 cgcgtgcgcg ggcactaccg cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc
    14761 accaccgtcg atgacgccat cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg
    14821 ccgccaccag tgtccacagt ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc
    14881 tatgctaaaa tgaagagacg gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc
    14941 actgccgccc aacgcgcggc ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg
    15001 gcggccatgc gggccgctcg aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc
    15061 aggcgacgag cggccgccgc agcagccgcg gccattagtg ctatgactca gggtcgcagg
    15121 ggcaacgtgt attgggtgcg cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc
    15181 cccccgcgca actagattgc aagaaaaaac tacttagact cgtactgttg tatgtatcca
    15241 gcggcggcgg cgcgcaacga agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag
    15301 gtcatcgcgc cggagatcta tggccccccg aagaaggaag agcaggatta caagccccga
    15361 aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg
    15421 gaactgctgc acgctaccgc gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa
    15481 cgtgttttgc gacccggcac caccgtagtc tttacgcccg gtgagcgctc cacccgcacc
    15541 tacaagcgcg tgtatgatga ggtgtacggc gacgaggacc tgcttgagca ggccaacgag
    15601 cgcctcgggg agtttgccta cggaaagcgg cataaggaca tgctggcgtt gccgctggac
    15661 gagggcaacc caacacctag cctaaagccc gtaacactgc agcaggtgct gcccgcgctt
    15721 gcaccgtccg aagaaaagcg cggcctaaag cgcgagtctg gtgacttggc acccaccgtg
    15781 cagctgatgg tacccaagcg ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa
    15841 cctgggctgg agcccgaggt ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc
    15901 gtgcagaccg tggacgttca gatacccact accagtagca ccagtattgc caccgccaca
    15961 gagggcatgg agacacaaac gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag
    16021 gcggtcgctg cggccgcgtc caagacctct acggaggtgc aaacggaccc gtggatgttt
    16081 cgcgtttcag ccccccggcg cccgcgcggt tcgaggaagt acggcgccgc cagcgcgcta
    16141 ctgcccgaat atgccctaca tccttccatt gcgcctaccc ccggctatcg tggctacacc
    16201 taccgcccca gaagacgagc aactacccga cgccgaacca ccactggaac ccgccgccgc
    16261 cgtcgccgtc gccagcccgt gctggccccg atttccgtgc gcagggtggc tcgcgaagga
    16321 ggcaggaccc tggtgctgcc aacagcgcgc taccacccca gcatcgttta aaagccggtc
    16381 tttgtggttc ttgcagatat ggccctcacc tgccgcctcc gtttcccggt gccgggattc
    16441 cgaggaagaa tgcaccgtag gaggggcatg gccggccacg gcctgacggg cggcatgcgt
    16501 cgtgcgcacc accggcggcg gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc
    16561 ctccttattc cactgatcgc cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc
    16621 ttgcaggcgc agagacactg attaaaaaca agttgcatgt ggaaaaatca aaataaaaag
    16681 tctggactct cacgctcgct tggtcctgta actattttgt agaatggaag acatcaactt
    16741 tgcgtctctg gccccgcgac acggctcgcg cccgttcatg ggaaactggc aagatatcgg
    16801 caccagcaat atgagcggtg gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa
    16861 tttcggttcc accgttaaga actatggcag caaggcctgg aacagcagca caggccagat
    16921 gctgagggat aagttgaaag agcaaaattt ccaacaaaag gtggtagatg gcctggcctc
    16981 tggcattagc ggggtggtgg acctggccaa ccaggcagtg caaaataaga ttaacagtaa
    17041 gcttgatccc cgccctcccg tagaggagcc tccaccggcc gtggagacag tgtctccaga
    17101 ggggcgtggc gaaaagcgtc cgcgccccga cagggaagaa actctggtga cgcaaataga
    17161 cgagcctccc tcgtacgagg aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc
    17221 gcccatggct accggagtgc tgggccagca cacacccgta acgctggacc tgcctccccc
    17281 cgccgacacc cagcagaaac ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc
    17341 tagccgcgcg tccctgcgcc gcgccgccag cggtccgcga tcgttgcggc ccgtagccag
    17401 tggcaactgg caaagcacac tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg
    17461 ccgacgatgc ttctgaatag ctaacgtgtc gtatgtgtgt catgtatgcg tccatgtcgc
    17521 cgccagagga gctgctgagc cgccgcgcgc ccgctttcca agatggctac cccttcgatg
    17581 atgccgcagt ggtcttacat gcacatctcg ggccaggacg cctcggagta cctgagcccc
    17641 gggctggtgc agtttgcccg cgccaccgag acgtacttca gcctgaataa caagtttaga
    17701 aaccccacgg tggcgcctac gcacgacgtg accacagacc ggtcccagcg tttgacgctg
    17761 cggttcatcc ctgtggaccg tgaggatact gcgtactcgt acaaggcgcg gttcacccta
    17821 gctgtgggtg ataaccgtgt gctggacatg gcttccacgt actttgacat ccgcggcgtg
    17881 ctggacaggg gccctacttt taagccctac tctggcactg cctacaacgc cctggctccc
    17941 aagggtgccc caaatccttg cgaatgggat gaagctgcta ctgctcttga aataaaccta
    18001 gaagaagagg acgatgacaa cgaagacgaa gtagacgagc aagctgagca gcaaaaaact
    18061 cacgtatttg ggcaggcgcc ttattctggt ataaatatta caaaggaggg tattcaaata
    18121 ggtgtcgaag gtcaaacacc taaatatgcc gataaaacat ttcaacctga acctcaaata
    18181 ggagaatctc agtggtacga aactgaaatt aatcatgcag ctgggagagt ccttaaaaag
    18241 actaccccaa tgaaaccatg ttacggttca tatgcaaaac ccacaaatga aaatggaggg
    18301 caaggcattc ttgtaaagca acaaaatgga aagctagaaa gtcaagtgga aatgcaattt
    18361 ttctcaacta ctgaggcgac cgcaggcaat ggtgataact tgactcctaa agtggtattg
    18421 tacagtgaag atgtagatat agaaacccca gacactcata tttcttacat gcccactatt
    18481 aaggaaggta actcacgaga actaatgggc caacaatcta tgcccaacag gcctaattac
    18541 attgctttta gggacaattt tattggtcta atgtattaca acagcacggg taatatgggt
    18601 gttctggcgg gccaagcatc gcagttgaat gctgttgtag atttgcaaga cagaaacaca
    18661 gagctttcat accagctttt gcttgattcc attggtgata gaaccaggta cttttctatg
    18721 tggaatcagg ctgttgacag ctatgatcca gatgttagaa ttattgaaaa tcatggaact
    18781 gaagatgaac ttccaaatta ctgctttcca ctgggaggtg tgattaatac agagactctt
    18841 accaaggtaa aacctaaaac aggtcaggaa aatggatggg aaaaagatgc tacagaattt
    18901 tcagataaaa atgaaataag agttggaaat aattttgcca tggaaatcaa tctaaatgcc
    18961 aacctgtgga gaaatttcct gtactccaac atagcgctgt atttgcccga caagctaaag
    19021 tacagtcctt ccaacgtaaa aatttctgat aacccaaaca cctacgacta catgaacaag
    19081 cgagtggtgg ctcccgggtt agtggactgc tacattaacc ttggagcacg ctggtccctt
    19141 gactatatgg acaacgtcaa cccatttaac caccaccgca atgctggcct gcgctaccgc
    19201 tcaatgttgc tgggcaatgg tcgctatgtg cccttccaca tccaggtgcc tcagaagttc
    19261 tttgccatta aaaacctcct tctcctgccg ggctcataca cctacgagtg gaacttcagg
    19321 aaggatgtta acatggttct gcagagctcc ctaggaaatg acctaagggt tgacggagcc
    19381 agcattaagt ttgatagcat ttgcctttac gccaccttct tccccatggc ccacaacacc
    19441 gcctccacgc ttgaggccat gcttagaaac gacaccaacg accagtcctt taacgactat
    19501 ctctccgccg ccaacatgct ctaccctata cccgccaacg ctaccaacgt gcccatatcc
    19561 atcccctccc gcaactgggc ggctttccgc ggctgggcct tcacgcgcct taagactaag
    19621 gaaaccccat cactgggctc gggctacgac ccttattaca cctactctgg ctctataccc
    19681 tacctagatg gaacctttta cctcaaccac acctttaaga aggtggccat tacctttgac
    19741 tcttctgtca gctggcctgg caatgaccgc ctgcttaccc ccaacgagtt tgaaattaag
    19801 cgctcagttg acggggaggg ttacaacgtt gcccagtgta acatgaccaa agactggttc
    19861 ctggtacaaa tgctagctaa ctacaacatt ggctaccagg gcttctatat cccagagagc
    19921 tacaaggacc gcatgtactc cttctttaga aacttccagc ccatgagccg tcaggtggtg
    19981 gatgatacta aatacaagga ctaccaacag gtgggcatcc tacaccaaca caacaactct
    20041 ggatttgttg gctaccttgc ccccaccatg cgcgaaggac aggcctaccc tgctaacttc
    20101 ccctatccgc ttataggcaa gaccgcagtt gacagcatta cccagaaaaa gtttctttgc
    20161 gatcgcaccc tttggcgcat cccattctcc agtaacttta tgtccatggg cgcactcaca
    20221 gacctgggcc aaaaccttct ctacgccaac tccgcccacg cgctagacat gacttttgag
    20281 gtggatccca tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc
    20341 cgtgtgcacc ggccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg
    20401 gccggcaacg ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct
    20461 ccagtgagca ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg
    20521 gcacctatga caagcgcttt ccaggctttg tttctccaca caagctcgcc tgcgccatag
    20581 tcaatacggc cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc
    20641 actcaaaaac atgctacctc tttgagccct ttggcttttc tgaccagcga ctcaagcagg
    20701 tttaccagtt tgagtacgag tcactcctgc gccgtagcgc cattgcttct tcccccgacc
    20761 gctgtataac gctggaaaag tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg
    20821 gactattctg ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc
    20881 acaaccccac catgaacctt attaccgggg tacccaactc catgctcaac agtccccagg
    20941 tacagcccac cctgcgtcgc aaccaggaac agctctacag cttcctggag cgccactcgc
    21001 cctacttccg cagccacagt gcgcagatta ggagcgccac ttctttttgt cacttgaaaa
    21061 acatgtaaaa ataatgtact agagacactt tcaataaagg caaatgcttt tatttgtaca
    21121 ctctcgggtg attatttacc cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt
    21181 tctgccgcgc atcgctatgc gccactggca gggacacgtt gcgatactgg tgtttagtgc
    21241 tccacttaaa ctcaggcaca accatccgcg gcagctcggt gaagttttca ctccacaggc
    21301 tgcgcaccat caccaacgcg tttagcaggt cgggcgccga tatcttgaag tcgcagttgg
    21361 ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg aacactatca
    21421 gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt
    21481 cctccgcgtt gctcagggcg aacggagtca actttggtag ctgccttccc aaaaagggcg
    21541 cgtgcccagg ctttgagttg cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg
    21601 tctgggcgtt aggatacagc gcctgcataa aagccttgat ctgcttaaaa gccacctgag
    21661 cctttgcgcc ttcagagaag aacatgccgc aagacttgcc ggaaaactga ttggccggac
    21721 aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga gatctgcacc acatttcggc
    21781 cccaccggtt cttcacgatc ttggccttgc tagactgctc cttcagcgcg cgctgcccgt
    21841 tttcgctcgt cacatccatt tcaatcacgt gctccttatt tatcataatg cttccgtgta
    21901 gacacttaag ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg
    21961 gctcgtgatg cttgtaggtc acctctgcaa acgactgcag gtacgcctgc aggaatcgcc
    22021 ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct
    22081 cgttcagcca ggtcttgcat acggccgcca gagcttccac ttggtcaggc agtagtttga
    22141 agttcgcctt tagatcgtta tccacgtggt acttgtccat cagcgcgcgc gcagcctcca
    22201 tgcccttctc ccacgcagac acgatcggca cactcagcgg gttcatcacc gtaatttcac
    22261 tttccgcttc gctgggctct tcctcttcct cttgcgtccg cataccacgc gccactgggt
    22321 cgtcttcatt cagccgccgc actgtgcgct tacctccttt gccatgcttg attagcaccg
    22381 gtgggttgct gaaacccacc atttgtagcg ccacatcttc tctttcttcc tcgctgtcca
    22441 cgattacctc tggtgatggc gggcgctcgg gcttgggaga agggcgcttc tttttcttct
    22501 tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg cgggctgggt gtgcgcggca
    22561 ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc gatacgccgc ctcatccgct
    22621 tttttggggg cgcccgggga ggcggcggcg acggggacgg ggacgacacg tcctccatgg
    22681 ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt
    22741 cccgactggc catttccttc tcctataggc agaaaaagat catggagtca gtcgagaaga
    22801 aggacagcct aaccgccccc tctgagttcg ccaccaccgc ctccaccgat gccgccaacg
    22861 cgcctaccac cttccccgtc gaggcacccc cgcttgagga ggaggaagtg attatcgagc
    22921 aggacccagg ttttgtaagc gaagacgacg aggaccgctc agtaccaaca gaggataaaa
    22981 agcaagacca ggacaacgca gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc
    23041 atggcgacta cctagatgtg ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg
    23101 ccattatctg cgacgcgttg caagagcgca gcgatgtgcc cctcgccata gcggatgtca
    23161 gccttgccta cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg
    23221 gcacatgcga gcccaacccg cgcctcaact tctaccccgt atttgccgtg ccagaggtgc
    23281 ttgccaccta tcacatcttt ttccaaaact gcaagatacc cctatcctgc cgtgccaacc
    23341 gcagccgagc ggacaagcag ctggccttgc ggcagggcgc tgtcatacct gatatcgcct
    23401 cgctcaacga agtgccaaaa atctttgagg gtcttggacg cgacgagaag cgcgcggcaa
    23461 acgctctgca acaggaaaac agcgaaaatg aaagtcactc tggagtgttg gtggaactcg
    23521 agggtgacaa cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc cactttgcct
    23581 acccggcact taacctaccc cccaaggtca tgagcacagt catgagtgag ctgatcgtgc
    23641 gccgtgcgca gcccctggag agggatgcaa atttgcaaga acaaacagag gagggcctac
    23701 ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg
    23761 aggagcgacg caaactaatg atggccgcag tgctcgttac cgtggagctt gagtgcatgc
    23821 agcggttctt tgctgacccg gagatgcagc gcaagctaga ggaaacattg cactacacct
    23881 ttcgacaggg ctacgtacgc caggcctgca agatctccaa cgtggagctc tgcaacctgg
    23941 tctcctacct tggaattttg cacgaaaacc gccttgggca aaacgtgctt cattccacgc
    24001 tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt ctatgctaca
    24061 cctggcagac ggccatgggc gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc
    24121 tgcagaaact gctaaagcaa aacttgaagg acctatggac ggccttcaac gagcgctccg
    24181 tggccgcgca cctggcggac atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg
    24241 gtctgccaga cttcaccagt caaagcatgt tgcagaactt taggaacttt atcctagagc
    24301 gctcaggaat cttgcccgcc acctgctgtg cacttcctag cgactttgtg cccattaagt
    24361 accgcgaatg ccctccgccg ctttggggcc actgctacct tctgcagcta gccaactacc
    24421 ttgcctacca ctctgacata atggaagacg tgagcggtga cggtctactg gagtgtcact
    24481 gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg caattcgcag ctgcttaacg
    24541 aaagtcaaat tatcggtacc tttgagctgc agggtccctc gcctgacgaa aagtccgcgg
    24601 ctccggggtt gaaactcact ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac
    24661 ctgaggacta ccacgcccac gagattaggt tctacgaaga ccaatcccgc ccgccaaatg
    24721 cggagcttac cgcctgcgtc attacccagg gccacattct tggccaattg caagccatca
    24781 acaaagcccg ccaagagttt ctgctacgaa agggacgggg ggtttacttg gacccccagt
    24841 ccggcgagga gctcaaccca atccccccgc cgccgcagcc ctatcagcag cagccgcggg
    24901 cccttgcttc ccaggatggc acccaaaaag aagctgcagc tgccgccgcc acccacggac
    24961 gaggaggaat actgggacag tcaggcagag gaggttttgg acgaggagga ggaggacatg
    25021 atggaagact gggagagcct agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa
    25081 acaccgtcac cctcggtcgc attcccctcg ccggcgcccc agaaatcggc aaccggttcc
    25141 agcatggcta caacctccgc tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac
    25201 cgtagatggg acaccactgg aaccagggcc ggtaagtcca agcagccgcc gccgttagcc
    25261 caagagcaac aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt
    25321 gcttgcttgc aagactgtgg gggcaacatc tccttcgccc gccgctttct tctctaccat
    25381 cacggcgtgg ccttcccccg taacatcctg cattactacc gtcatctcta cagcccatac
    25441 tgcaccggcg gcagcggcag cggcagcaac agcagcggcc acacagaagc aaaggcgacc
    25501 ggatagcaag actctgacaa agcccaagaa atccacagcg gcggcagcag caggaggagg
    25561 agcgctgcgt ctggcgccca acgaacccgt atcgacccgc gagcttagaa acaggatttt
    25621 tcccactctg tatgctatat ttcaacagag caggggccaa gaacaagagc tgaaaataaa
    25681 aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg aagatcagct
    25741 tcggcgcacg ctggaagacg cggaggctct cttcagtaaa tactgcgcgc tgactcttaa
    25801 ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc tccagcggcc
    25861 acacccggcg ccagcacctg tcgtcagcgc cattatgagc aaggaaattc ccacgcccta
    25921 catgtggagt taccagccac aaatgggact tgcggctgga gctgcccaag actactcaac
    25981 ccgaataaac tacatgagcg cgggacccca catgatatcc cgggtcaacg gaatccgcgc
    26041 ccaccgaaac cgaattctct tggaacaggc ggctattacc accacacctc gtaataacct
    26101 taatccccgt agttggcccg ctgccctggt gtaccaggaa agtcccgctc ccaccactgt
    26161 ggtacttccc agagacgccc aggccgaagt tcagatgact aactcagggg cgcagcttgc
    26221 gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt ataactcacc tgacaatcag
    26281 agggcgaggt attcagctca acgacgagtc ggtgagctcc tcgcttggtc tccgtccgga
    26341 cgggacattt cagatcggcg gcgccggccg tccttcattc acgcctcgtc aggcaatcct
    26401 aactctgcag acctcgtcct ctgagccgcg ctctggaggc attggaactc tgcaatttat
    26461 tgaggagttt gtgccatcgg tctactttaa ccccttctcg ggacctcccg gccactatcc
    26521 ggatcaattt attcctaact ttgacgcggt aaaggactcg gcggacggct acgactgaat
    26581 gttaagtgga gaggcagagc aactgcgcct gaaacacctg gtccactgtc gccgccacaa
    26641 gtgctttgcc cgcgactccg gtgagttttg ctactttgaa ttgcccgagg atcatatcga
    26701 gggcccggcg cacggcgtcc ggcttaccgc ccagggagag cttgcccgta gcctgattcg
    26761 ggagtttacc cagcgccccc tgctagttga gcgggacagg ggaccctgtg ttctcactgt
    26821 gatttgcaac tgtcctaacc ttggattaca tcaagatctt tgttgccatc tctgtgctga
    26881 gtataataaa tacagaaatt aaaatatact ggggctccta tcgccatcct gtaaacgcca
    26941 ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc tggtactttt aacatctctc
    27001 cctctgtgat ttacaacagt ttcaacccag acggagtgag tctacgagag aacctctccg
    27061 agctcagcta ctccatcaga aaaaacacca ccctccttac ctgccgggaa cgtacgagtg
    27121 cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa accagacttt ttccggacag
    27181 acctcaataa ctctgtttac cagaacagga ggtgagctta gaaaaccctt agggtattag
    27241 gccaaaggcg cagctactgt ggggtttatg aacaattcaa gcaactctac gggctattct
    27301 aattcaggtt tctctagaaa tggacggaat tattacagag cagcgcctgc tagaaagacg
    27361 cagggcagcg gccgagcaac agcgcatgaa tcaagagctc caagacatgg ttaacttgca
    27421 ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc aaagtcacct acgacagtaa
    27481 taccaccgga caccgcctta gctacaagtt gccaaccaag cgtcagaaat tggtggtcat
    27541 ggtgggagaa aagcccatta ccataactca gcactcggta gaaaccgaag gctgcattca
    27601 ctcaccttgt caaggacctg aggatctctg cacccttatt aagaccctgt gcggtctcaa
    27661 agatcttatt ccctttaact aataaaaaaa aataataaag catcacttac ttaaaatcag
    27721 ttagcaaatt tctgtccagt ttattcagca gcacctcctt gccctcctcc cagctctggt
    27781 attgcagctt cctcctggct gcaaactttc tccacaatct aaatggaatg tcagtttcct
    27841 cctgttcctg tccatccgca cccactatct tcatgttgtt gcagatgaag cgcgcaagac
    27901 cgtctgaaga taccttcaac cccgtgtatc catatgacac ggaaaccggt cctccaactg
    27961 tgccttttct tactcctccc tttgtatccc ccaatgggtt tcaagagagt ccccctgggg
    28021 tactctcttt gcgcctatcc gaacctctag ttacctccaa tggcatgctt gcgctcaaaa
    28081 tgggcaacgg cctctctctg gacgaggccg gcaaccttac ctcccaaaat gtaaccactg
    28141 tgagcccacc tctcaaaaaa accaagtcaa acataaacct ggaaatatct gcacccctca
    28201 cagttacctc agaagcccta actgtggctg ccgccgcacc tctaatggtc gcgggcaaca
    28261 cactcaccat gcaatcacag gccccgctaa ccgtgcacga ctccaaactt agcattgcca
    28321 cccaaggacc cctcacagtg tcagaaggaa agctagccct gcaaacatca ggccccctca
    28381 ccaccaccga tagcagtacc cttactatca ctgcctcacc ccctctaact actgccactg
    28441 gtagcttggg cattgacttg aaagagccca tttatacaca aaatggaaaa ctaggactaa
    28501 agtacggggc tcctttgcat gtaacagacg acctaaacac tttgaccgta gcaactggtc
    28561 caggtgtgac tattaataat acttccttgc aaactaaagt tactggagcc ttgggttttg
    28621 attcacaagg caatatgcaa cttaatgtag caggaggact aaggattgat tctcaaaaca
    28681 gacgccttat acttgatgtt agttatccgt ttgatgctca aaaccaacta aatctaagac
    28741 taggacaggg ccctcttttt ataaactcag cccacaactt ggatattaac tacaacaaag
    28801 gcctttactt gtttacagct tcaaacaatt ccaaaaagct tgaggttaac ctaagcactg
    28861 ccaaggggtt gatgtttgac gctacagcca tagccattaa tgcaggagat gggcttgaat
    28921 ttggttcacc taatgcacca aacacaaatc ccctcaaaac aaaaattggc catggcctag
    28981 aatttgattc aaacaaggct atggttccta aactaggaac tggccttagt tttgacagca
    29041 caggtgccat tacagtagga aacaaaaata atgataagct aactttgtgg accacaccag
    29101 ctccatctcc taactgtaga ctaaatgcag agaaagatgc taaactcact ttggtcttaa
    29161 caaaatgtgg cagtcaaata cttgctacag tttcagtttt ggctgttaaa ggcagtttgg
    29221 ctccaatatc tggaacagtt caaagtgctc atcttattat aagatttgac gaaaatggag
    29281 tgctactaaa caattccttc ctggacccag aatattggaa ctttagaaat ggagatctta
    29341 ctgaaggcac agcctataca aacgctgttg gatttatgcc taacctatca gcttatccaa
    29401 aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca agtttactta aacggagaca
    29461 aaactaaacc tgtaacacta accattacac taaacggtac acaggaaaca ggagacacaa
    29521 ctccaagtgc atactctatg tcattttcat gggactggtc tggccacaac tacattaatg
    29581 aaatatttgc cacatcctct tacacttttt catacattgc ccaagaataa agaatcgttt
    29641 gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa tttcgaatca tttttcattc
    29701 agtagtatag ccccaccacc acatagctta tacagatcac cgtaccttaa tcaaactcac
    29761 agaaccctag tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct
    29821 ccccggctgg ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata
    29881 ttccacacgg tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc
    29941 agctcactta agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc
    30001 ggttgcttaa cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg
    30061 tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc
    30121 tccgtcctgc aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc
    30181 agcataaggc gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca
    30241 cagtaactgc agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat
    30301 ccaaagctca tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag
    30361 attaagtggc gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg
    30421 taattcacca cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc
    30481 atcctaaacc agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg
    30541 gaacaatgac agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata
    30601 tcaatgttgg cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc
    30661 cgcgttagaa ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg
    30721 cagggaagac ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc
    30781 agcggatgat cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc
    30841 ctactgtacg gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat
    30901 ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga
    30961 tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc
    31021 tctcaaagca tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc
    31081 tgccctgata acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt
    31141 ctgcgagtca cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt
    31201 ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg
    31261 tggcgtggtc aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa
    31321 tggcttccaa aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag
    31381 ggtgaatctc ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc
    31441 gccaccttct caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa
    31501 tctgctccag agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc
    31561 aggttcctca cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc
    31621 ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc
    31681 ggccacttcc ccgccaggaa ccttgacaaa agaacccaca ctgattatga cacgcatact
    31741 cggagctatg ctaaccagcg tagccccgat gtaagctttg ttgcatgggc ggcgatataa
    31801 aatgcaaggt gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt
    31861 agtcatgctc atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca
    31921 tttttctctc aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa
    31981 catttaaaca ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg
    32041 gactacggcc atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac
    32101 cgacagctcc tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg
    32161 attcacatcg gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag
    32221 gcgtagagac aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa
    32281 cacataaaca cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac
    32341 aacatacagc gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa
    32401 cctattaaaa aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg
    32461 gccaagtgca gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa
    32521 aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca
    32581 acttcctcaa atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac
    32641 tacaattccc aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc
    32701 cacgccccgc gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa
    32761 ataaggtata ttattgatga tgttaattaa tttaaatccg catgcgatat cgagctctcc
    32821 cgggaattcg gatctgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
    32881 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
    32941 catcagggac agcttcacgg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    33001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    33061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    33121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    33181 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    33241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    33301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    33361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    33421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    33481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    33541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    33601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    33661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatca atctaaagta
    33721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    33781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    33841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    33901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    33961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    34021 gttcgccagt taatagtttg cgcaacgttg ttgccattgn tgcaggcatc gtggtgtcac
    34081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    34141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    34201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    34261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    34321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
    34381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    34441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    34501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    34561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    34621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    34681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    34741 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    34801 ttcgtcttca aggatccgaa ttcccgggag agctcgatat cgcatgcgga tttaaattaa
    34861 ttaa
//

质粒菌株产品操作说明书
一、扩增流程
         收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
         1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              ①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
              ②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
              ③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
              ④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
              ⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
              ⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
        2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
              四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
        3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
              穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
        4、菌落平板（冰袋运输，存于4℃，保质期7天）
              直接挑取单菌落至液体培养基中。
        5、液体质粒（冰袋运输，存于-20℃，保质期90天）
              单独提取的液体质粒收到后可直接使用。
        6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。

二、转化图片

P2292/pENTR223-RPS2人源基因质粒

P2326/pENTR223-ATP5PO人源基因质粒

P2327/pENTR223-SLC25A10人源基因质粒

P2336/pENTR223-CCDC24人源基因质粒

P2337/pENTR223-BAT2L人源基因质粒

P2338/pENTR223-RSPH14人源基因质粒

P2339/pENTR223-ABHD5人源基因质粒

P2340/pENTR223-NONO人源基因质粒

P2341/pENTR223-MYOZ1人源基因质粒

P2342/pENTR223-STX5人源基因质粒

P2343/pENTR223-GK5人源基因质粒