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- 90048-1mg
- 美国
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
Cyanine 680 succinimidyl ester
- CAS号：
1
- 保质期：
6个月
- 供应商：
Biotium
- 保存条件：
-20°C
Biotium offers high quality amine-reactive succinimidyl ester (SE, or NHS-Ester) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters. Also see our CF® Dye Succinimidyl Esters, available with more than 20 bright and photostable CF® Dyes.
Cyanine 555
Cyanine 555 is structurally identical to Cy®3 from GE Healthcare.
- λEx/λEm (water) = 555/565 nm
- Dark red solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C41H56N4O10S2
- MW: 829
Cyanine 647
Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.
- λEx/λEm (water) = 650/665 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C43H58N4O10S2
- MW: 855
Cyanine 680
Cyanine 680 is structurally identical to Cy®5.5 from GE Healthcare.
- λEx/λEm (water) = 681/698 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C63H92N6O16S4
- MW: 1318
Cyanine 750
Cyanine 750 is structurally identical to Cy®7 from GE Healthcare.
- λEx/λEm = 755/777 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C45H60N4O10S2
- MW: 881
Cy3, Cy5, Cy5.5, and Cy7 are registered trademarks of GE Healthcare.
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文献和实验SE 活性部分远低于50%（AlexaFluor488微量标记试剂盒产品信息，Invitrogen）。相反，所有Biotium 的CF染料具有相对稳定的胺活性的SE基团型，这比大部分AlexaFluor 染料的SE更耐水解。总体来说，CF染料SE产品一贯的给出更高的标记效率，提供给用户更好的使用价值。选择方案：蓝色（350-450nm处激发） CF 350、Alexa Fluor 350、AMCA等----亮蓝和紫外光激发CF350是类似于Alexa Fluor 350和传统荧光染料AMCA的蓝色
与具有Fc受体的细胞结合的情况，或需要封闭内源性免疫球蛋白的时候。 对于二抗所耦联的探针的选择，主要取决于具体的实验。对于Western blot，最常用的探针是辣根过氧化酶（HRP），碱性磷酸酶（AP）的使用也很多，但不如HRP使用广泛。此外，IRDye (如 IRDye 680、IRDye 800) and Alexa Fluor dye (如Alexa 680 ) 等系列染料也偶尔应用于 western blot当中。通过统计分析发现，来自于Amersham
(MCA, AMC)Alexa Fluor 488Cy2 or Fluorescein (FITC, FAM)Alexa Fluor 555Cy3 or Tetramethylrhodamine (TRMRA)Alexa Fluor 568Rhodamine RedAlexa Fluor 594Texas RedAlexa Fluor 647Cy5Alexa Fluor 680Cy5.5Alexa Fluor 750Cy7荧光分子颜色激发波长发射波长Alexa Fluor 350Blue346 nm
