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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
25696-60-0
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
3) (60 mM)MALDI matrix A Prepare a saturated solution of {alpha}-cyano-4-hydroxy-cinnamic acid in:Ethanol (45% [v/v])H2O (45% [v/v])10% formic acid (10% [v/v]).Store in the dark at room temperature.Nickel-nitrilotriacetic acid (Ni-NTA) resinPhosphoprotein
Phosphoamino acid analysis:Mark Kamps's method
or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting
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