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- ChemiScreen™
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- HTS022M
- 2025年07月07日
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文献和实验Characterization of Melanocortin Receptors
CHO‐K1 cells expressing a recombinant melanocortin receptor (MC3R, MC4R and MC5R membranes are available from Perkin Elmer Life Sciences) Culture medium (see recipe
-and sphingolipid-rich plasma membrane microdomains that were resistant to solubilization in the cold non-ionic detergent Triton X-100. Such detergent-insoluble membrane domains were of low buoyant density and could be readily purified on sucrose equilibrium density
Preparation of Brain Membrane Fractions
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedures should be done at 4ºC using precooled reagents. For rat samples, immediately remove brain from the cranium into ice cold dissection buffer (50 mM Tris-acetate (TA
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