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| DiscoverX 提供具有化学发光和荧光读数的检测试剂,以满足您的药物发现计划的不同需求。DiscoverX 的化学发光检测基于专有的酶片段互补 (EFC) 技术,而荧光检测基于激活的荧光染料。这些检测试剂针对各种应用进行了优化,例如检测基于细胞的蛋白质-蛋白质相互作用、化合物-蛋白质结合、易位和第二信使测定。 DiscoverX 的检测试剂专为 DiscoverX 细胞系设计,包括 cAMP Hunter™、InCELL Hunter™ 或 PathHunter® 细胞系、InCELL 检测和 ProLabel®/ProLink™ (PL/PK) 表达或全细胞内的克隆载体。 |
产品亮点
- 灵敏的检测和可重现的结果提供您可以信赖的可靠检测
- 简单的一步添加方案,非常适合多种基础研究和药物发现应用
- 可从 96 孔扩展到 4146 孔格式,与高通量筛选或小型化兼容
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文献和实验BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
) available from Invitrogen(2). The amount of BLOCK-iT™ Fluorescent Oligo to use depends on the growth rate and transfection efficiency of the mammalian cells. If you are transfecting your mammalian cell line for the first time, we recommend
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
and a preparation of highly competent E. coli is transformed. The library is then grown as a pool and a preparation of plasmids from the pool is made. The DNA of the pool is transfected into an avian or mammalian packaging cell line to produce a library of virus
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
from the pool is made. The DNA of the pool is transfected into an avian or mammalian packaging cell line to produce a library of virus particles. The library is injected into an area to be mapped. Infected cells are detected histochemically and each infected
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