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- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Sucrase (Maltase from yeast)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
9001-42-7
- 保存条件：
2-8℃
- 规格：
300U
High purity Sucrase (from yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Sucrase (E-SUCR) and Fructanase (E-FRMXLQ or E-FRMXPD) are used in the measurement of fructan in foods according to Chinese GB Standard 5009.255-2016 and in the AOAC method 2016.06.
| Content: | 300 Units (on sucrose) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Supplied as a lyophilised powder |
| Physical Form: | Powder |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Sucrase/Invertase |
| EC Number: | 3.2.1.20 |
| CAZy Family: | GH13 |
| CAS Number: | 9001-42-7 |
| Synonyms: | alpha-glucosidase; alpha-D-glucoside glucohydrolase |
| Source: | Yeast |
| Molecular Weight: | 62,000 |
| Expression: | Purified from Yeast |
| Specificity: | Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose. |
| Specific Activity: | ~ 20 U/mg (30oC, pH 6.8 on sucrose) |
| Unit Definition: | One Unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 30oC. |
| Temperature Optima: | 30oC |
| pH Optima: | 6.8 |
| Application examples: | Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries. |
| Method recognition: | AOAC Method 2016.06 and GB Standard 5009.255-2016 |
如需蔗糖酶[麦芽糖发酵]产品完整说明书，
请向上海经科客服索取！
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文献和实验1.The molecular basis of the effect of temperature on enzyme activity.
Daniel, R. M., Peterson, M. E., Danson, M. J., Price, N. C., Kelly, S. M., Monk, C R., Weinberg, C S., Oudshoorn, M. L. & Lee, C. K. (2010). Biochem. J, 425, 353-360.
蔗糖酶 sucrase 将蔗糖水解成 D-果糖和 D-葡萄糖的β-D-果糖苷酶的一种，又称为转化酶（invertase）或蔗糖酶（saccharase） EC 3．2．1．26。除广泛分布于微生物、植物外，类似的活性也在动物的消化液中发现，从链孢霉提纯获得的酶除作用蔗糖外，还可作用具有β-呋喃果糖苷键的物质。例如棉子糖、水苏糖等的寡糖，尤其也能同样地作用具有作为糖苷配基的甲基等的烷基、苯基等丙烯基的配糖体。一般微生物的蔗糖酶也催化从蔗糖将β-果糖苷基转移到其他糖、醇、酚上的反应
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
实验步骤 1. Inoculate 5 ml YPD medium placed in a 10-20 ml culture tube with Yeast carrying desired plasmid and grow at 30°C with agitation for 20-24 h. 2. Pellet 1-3 ml yeast culture (use 7 cells) by centrifugation at 4,000×g
，路德酵母、酿酒酵母等常被用作遗传学的材料。 细孢形态为 8微米左右的椭圆形和球形。细胞组成，有由酵母甘露聚糖，酵母胶质构成的半纤维素性的细胞壁，细胞内有液胞和酵母糖原、脂粒、核及嗜碱粒。蛋白质可达干物量的 30-70％。尤其是有大量的 RNA存在。维生素，则含有丰富的核黄素，硫胺素和烟酸等。此外，麦角甾醇和谷胱甘肽非常多。由于从培养酵母可得到大量酶样品，因此，很多都促进了生物化学的发展，为蔗糖酶，组织蛋白酶、接触酶、发酵酶系（酒化酶）成分、细胞色素等。平常在植物体表面或动物体（肠道
