- ￥3573
- Megazyme
- 爱尔兰
- E-SUCRBG
- 2025年12月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Sucrase plus β-Galactosidase
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
9001-42-7
- 保存条件：
-20℃
- 规格：
170 U
蔗糖酶β-半乳糖苷酶介绍：
中文名称：蔗糖酶加β-半乳糖苷酶
英文名称：Sucrase plus β-Galactosidase
货号：E-SUCRBG
规格：170 U Sucrase plus 3,000 U β-Galactosidase
价格：3573元
High purity Sucrase (maltase; Yeast) / β-Galactosidase (Asper-gillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 170 Units Sucrase plus 3,000 Units β-Galactosidase |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Freeze-dried powder |
| Physical Form: | Powder |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | β-Galactosidase, Sucrase/Invertase |
| EC Number: | Sucrase: 3.2.1.20, β-Galactosidase: 3.2.1.23 |
| CAZy Family: | GH13, GH35 |
| CAS Number: | Sucrase: 9001-42-7, β-Galactosidase: 9031-11-2 |
| Synonyms: | Sucrase: alpha-glucosidase; alpha-D-glucoside glucohydrolase, β-Galactosidase: beta-galactosidase; beta-D-galactoside galactohydrolase |
| Source: | Sucrase: Yeast, β-Galactosidase: Asper-gillus niger |
| Molecular Weight: | 57,750 |
| Concentration: | Sucrase (170 U) / β-Galactosidase (3,000 U) |
| Expression: | Sucrase from Yeast. β-Galactosidase from Asper-gillus niger. |
| Specificity: | This enzyme mixture gives complete hydrolysis of sucrose, maltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf. |
| Specific Activity: | Sucrase (170 U) / β-Galactosidase (3,000 U) |
| Unit Definition: | Sucrase: One Unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium phosphate buffer (100 mM), pH 6.8 at 40oC. β-Galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 5 |
| Application examples: | Used for removal of sucrose, maltose and lactose in the measurement of dietary fiber. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1.Production and prebiotic properties of oligofructans from sugarcane juice fermentation by Bacillus subtilis TISTR 001.
Ninchan, B. & Noidee, C. (2021). 3 Biotech, 11(5), 1-11.
2.Efficacy of reducing sugar and phenol–sulfuric-acid assays for analysis of soluble carbohydrates in feedstuffs.
Hall, M. B. (2013). Animal Feed Science and Technology, 185(1), 94-100.
蔗糖酶 sucrase 将蔗糖水解成 D-果糖和 D-葡萄糖的β-D-果糖苷酶的一种，又称为转化酶（invertase）或蔗糖酶（saccharase） EC 3．2．1．26。除广泛分布于微生物、植物外，类似的活性也在动物的消化液中发现，从链孢霉提纯获得的酶除作用蔗糖外，还可作用具有β-呋喃果糖苷键的物质。例如棉子糖、水苏糖等的寡糖，尤其也能同样地作用具有作为糖苷配基的甲基等的烷基、苯基等丙烯基的配糖体。一般微生物的蔗糖酶也催化从蔗糖将β-果糖苷基转移到其他糖、醇、酚上的反应
了一个多克隆位点,但并没有破坏lacZ的阅读框架,不影响其正常功能。E. coli DH5α菌株带有β-半乳糖苷酶C端部分序列的编码信息。在各自独立的情况下,pBS和DH5α编码的β-半乳糖苷酶的片段都没有酶活性。但在pBS和DH5α融为一体时可形成具有酶活性的蛋白质。这种lacZ基因上缺失近操纵基因区段的突变体与带有完整的近操纵基因区段的β-半乳糖苷酸阴性突变体之间实现互补的现象叫α-互补。由α-互补产生的Lac+ 细菌较易识别，它在生色底物X-gal(5-溴-4氯-3-吲哚-β-D-半乳糖
，这些糖首先在口腔被唾液中的淀粉酶(α-amylase)部分水解α-1，4糖苷键(α-1.4glycosidic bond)，进而在小肠被胰液中的淀粉酶进一步水解生成麦芽糖，异麦芽糖和含4个糖基的临界糊精(α-dextrins)，最终被小肠粘膜刷毛缘的麦芽糖酶(maltase)、乳糖酶(lactase)和蔗糖酶(sucrase)水解为葡萄糖(glucose)、果糖(fructose)、半乳糖(galatose)，这些单糖可吸收入小肠细胞。此吸收过程是一个主动耗能的过程，由特定载体完成，同时伴有Na
