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- Megazyme
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- E-PULBL
- 2026年06月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Pullulanase M2 (B. licheniformis)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
9075-68-7
- 保存条件：
2-8℃
- 规格：
2000U
普鲁兰酶介绍：
中文名称：普鲁兰酶M2[地衣芽孢杆菌]
英文名称：Pullulanase M2 (Bacillus licheniformis)
货号：E-PULBL
规格：2,000 Units
价格：3701元
类别：碳水化合物活性酶
High purity Pullulanase M2 (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 2,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Pullulanase/Limit-dextrinase |
| EC Number: | 3.2.1.41 |
| CAZy Family: | GH13 |
| CAS Number: | 9075-68-7 |
| Synonyms: | pullulanase; pullulan 6-alpha-glucanohydrolase |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 113,000 |
| Concentration: | Supplied at ~ 1000 U/mL |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen. |
| Specific Activity: | ~ 78 U/mg (40oC, pH 5.0 on pullulan) |
| Unit Definition: | One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC. |
| Temperature Optima: | 55oC |
| pH Optima: | 5 |
| Application examples: | Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups. |
普鲁兰酶说明书(PDF)请向客服索取！
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- 作者
- 内容
- 询问日期
文献和实验1.Sequential starch modification by branching enzyme and 4-α-glucanotransferase improves retention of curcumin in starch-alginate beads.
Wang, Y., Pang, C., Mohammad-Beigi, H., Li, X., Wu, Y., Lin, M. K. T. H., Bai, Y., Moller, M. S. & Svensson, B. (2024). Carbohydrate Polymers, 323, 121387.
2.Impact of Starch Binding Domain Fusion on Activities and Starch Product Structure of 4-α-Glucanotransferase.
Wang, Y., Wu, Y., Christensen, S. J., Janeček, Š., Bai, Y., Møller, M. S. & Svensson, B. (2023). Molecules, 28(3), 1320.
3.Structural, physicochemical and digestive properties of rice starch modified by preheating and pullulanase treatments.
Geng, D. H., Zhang, X., Zhu, C., Wang, C., Cheng, Y. & Tang, N. (2023). Carbohydrate Polymers, 313, 120866.
4.Sequential maltogenic α-amylase and branching enzyme treatment to modify granular corn starch.
Zhong, Y., Herburger, K., Kirkensgaard, J. J. K., Khakimov, B., Hansen, A. R. & Blennow, A. (2021). Food Hydrocolloids, 120, 106904.
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
Elabscience 巨噬细胞三大功能及检测方案全梳理，看完少走90%弯路！
-α）、白细胞介素-1β（IL-1β）、IL-6、IL-12和IL-23等，这些因子可激活免疫细胞并促进炎症反应。 M2型（替代活化）巨噬细胞则主要分泌抗炎细胞因子，如IL-10和转化生长因子-β（TGF-β），发挥抑制炎症、促进组织修复的作用。 细胞因子通过组成型分泌途径（经由高尔基体→循环内体→细胞膜）或非经典途径（如IL-1β通过焦亡相关的膜孔释放）输出至胞外。 2. 分泌趋化因子 巨噬细胞可分泌多种趋化因子，主要功能是招募循环中的白细胞迁移至炎症或感染部位。 巨噬细胞产生的关键趋化
