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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Pectate lyase (Asper-gillus sp.)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
9015-75-2
- 保存条件：
-20℃
- 规格：
7,000 Units
High purity Pectate lyase (Asper-gillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 7,000 Units in 50% aqueous glycerol |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Pectate Lyase |
| EC Number: | 4.2.2.2 |
| CAZy Family: | PL1 |
| CAS Number: | 9015-75-2 |
| Synonyms: | pectate lyase; (1→4)-alpha-D-galacturonan lyase |
| Source: | Asper-gillus sp. |
| Molecular Weight: | 45,000 |
| Concentration: | Supplied at ~ 1,400 U/mL |
| Expression: | Purified from Asper-gillus sp. |
| Specificity: | Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends. |
| Specific Activity: | ~ 180 U/mg (40oC, pH 8.0 on polygalacturonic acid) |
| Unit Definition: | One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40oC. |
| Temperature Optima: | 55oC |
| pH Optima: | 8 |
| Application examples: | Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research. |
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文献和实验1.Xylobiose treatment triggers a defense-related response and alters cell wall composition.
Dewangan, B. P., Gupta, A., Sah, R. K., Das, S., Kumar, S., Bhattacharjee, S. & Pawar, P. A. M. (2023). Plant Molecular Biology, 113(6), 383-400.
2.Transcellular progression of infection threads in Medicago truncatula roots is controlled by locally confined cell wall modifications.
Su, C., Zhang, G., Rodriguez-Franco, M., Wietschorke, J., Liang, P., Yang, W., Uhler, L., Li, X. & Ott, T. (2022). BioRxiv, 2022-07.
Raimundo, S. C., Sørensen, I., Tinaz, B., Ritter, E., Rose, J. K. & Domozych, D. S. (2018). Plant Methods, 14(1), 18.
Kim, J. S. & Daniel, G. (2017). Trees, 1-22.
5.Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen.
Şahin, A. A., Aslım, B., Tan, S., Alan, Ş. & Pınar, N. M. (2017). Turkish Journal of Biochemistry, 43(4).
【信息】Northstar BioProducts（NSBP）
, (Streptococcus dysgalactiae), EC 4.2.2 0.5 unit Hyaluronate lyase (Streptococci Hyaluronidase, Hyaluronidase, Mucinase) Lyophilized powder. Activity: 0.5 units/vial. M.W.: 125,000 ± 10,000 (gel filtration) 100810-1 Keratanase, (Pseudomonas sp
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
-MF[80]Candida rugosa LIP2 lipaseS, 2.3 mg/L, α-MF, active463Candida rugosa lip4 lipaseS, 100mg/L, α-MF, active459Fusarium solani pectate lyase (pelC)S, 1 mg/L, PHO1[81]Fusarium solani pectate lyase (pelD)S, native[82]Geotrichum candidum lipase
P. , Lin, H. C. , Wang, S. S. , Callaway, J. , and Wilcox, G. ( 1 987) Characterization of the Erwinia carotovora peLB gene and its product pectate lyase. J . BacterioL 16 9, 4379-4383. 34. Yanisch-Perron, C. , Vieira
