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- 2025年12月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
exo-Inulinase (A. niger) (Recombinant)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
37288-56-5 / 9001-57-4
- 保存条件：
2-8℃
- 规格：
5,000 Units
外切菊粉酶：
中文名称：菊粉外切酶[黑曲菌]
英文名称：exo-Inulinase (Asper-gillus niger)
货号：E-EXOIAN
CAS:37288-56-5 ; 9001-57-4
规格：5,000 Units at 40oC
价格：2907元
类别：碳水化合物活性酶
High purity recombinant exo-Inulinase (Asper-gillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 5,000 Units at 40oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | β-Fructosidase |
| EC Number: | 3.2.1.80, 3.2.1.26 |
| CAZy Family: | GH32 |
| CAS Number: | 37288-56-5 |
| Synonyms: | fructan beta-fructosidase; beta-D-fructan fructohydrolase, beta-fructofuranosidase; beta-D-fructofuranoside fructohydrolase |
| Source: | Asper-gillus niger |
| Molecular Weight: | 58,400 |
| Concentration: | Supplied at ~ 2,000 U/mL (40oC, pH 4.5 on kestose) |
| Expression: | Recombinant from Asper-gillus niger |
| Specificity: | EC 3.2.1.80: Hydrolysis of terminal, non-reducing (2,1)- and (2,6)-linked β-D-fructofuranose residues in fructans. EC 3.2.1.26: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
| Specific Activity: | ~ 2,000 U/mg (60oC, pH 4.5 on kestose); ~ 1,000 U/mg (40oC, pH 4.5 on kestose) |
| Unit Definition: | One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one µmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC. |
| Temperature Optima: | 60oC |
| pH Optima: | 4.5 |
| Application examples: | Applications established in food industry for fructose syrup production and in the diagnostics industry for the measurement of fructans and inulins. |
外切菊粉酶说明书(PDF)请向客服索取！
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文献和实验1.Microbial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans.
Han, N. D., Cheng, J., Delannoy-Bruno, O., Webber, D., Terrapon, N., Henrissat, B., et al. (2022). Cell, 185(14), 2495-2509.
2.The inulin hydrolysis by recombinant exo-inulinases: determination the optimum temperatures and activation energies.
Miłek, J. (2021). Journal of Thermal Analysis and Calorimetry, 1-7.
3.Energy-Saving LED Light Affects the Efficiency of the Photosynthetic Apparatus and Carbohydrate Content in Gerbera jamesonii Bolus ex Hook. f. Axillary Shoots Multiplied In Vitro.
Cioć, M., Tokarz, K., Dziurka, M. & Pawłowska, B. (2021). Biology, 10(10), 1035.
亦可写成inulinase。是作用于菊粉水解其β -2。 1-果糖苷键生成 D-果糖的一种β -2， 1-果聚糖酶（β -2， 1-fructanase）。 EC3． 2． 1． 7。已知存在于蜗牛、菊芋、酵母、黑曲霉等。从菊芋的块茎中提纯的菊粉酶不作用蔗糖或细菌果聚糖，只作用于菊粉和它的寡糖，这时除生成 D-果糖外，还生成蔗糖。
)通过质膜。 Exon (外显子)：割裂基因中在成熟mRNA 产物中表达的任何片段。 Exonucleases (核酸外切酶)：从核酸链中每次从一头切割一个核苷酸，可能特异性切割DNA 或者RNA 的5￠或者3￠端。 Expression vector (表达载体)：设计好的克隆载体，使编码序列插入特定的位点，能够转录和翻译成蛋白质。 Extranuclear genes (核外基因)：核外的、定位在细胞器，如线粒体或叶绿体中的基因。
the ChIP?exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion followed by high?throughput sequencing. ChIP?exo allows identification of a nearly complete set of the binding locations of DNA?binding proteins
