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- 2025年12月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
β-Glucuronidase (Escherichia coli)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
9001-45-0
- 保存条件：
4℃
- 规格：
500,000 Units
β-葡萄糖醛酸酶β-Glucuronidase介绍：
中文名称：β-葡萄糖醛酸酶[大肠杆菌]
英文名称：β-Glucuronidase (E. coli)
货号：E-BGLAEC
规格：500,000 Units
价格：4595元
类别：碳水化合物活性酶
High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 500,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In solution (Tris.HCl/NaCl/EDTA) |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | β-Glucuronidase |
| EC Number: | 3.2.1.31 |
| CAZy Family: | GH2 |
| CAS Number: | 9001-45-0 |
| Synonyms: | β-D-glucuronoside glucuronosohydrolase; GUS |
| Source: | Escherichia coli |
| Molecular Weight: | 82,600 |
| Concentration: | Supplied at ~ 250 kU/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates. |
| Specific Activity: | ~ 15,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide); ~ 50 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide) |
| Unit Definition: | 30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37oC. 110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37oC., monitored at 410 nm. |
| Temperature Optima: | 37oC |
| pH Optima: | 6.8 |
β-葡萄糖醛酸酶β-Glucuronidase说明书(PDF)请向客服索取！
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文献和实验1.Dried urine spots as sampling technique for multi-mycotoxin analysis in human urine.
Schmidt, J., Lindemann, V., Olsen, M., Cramer, B. & Humpf, H. U. (2021). Mycotoxin Research, 37(2), 129-140.
Tan, X., Li, X., Chen, L., Xie, F., Li, L. & Huang, J. (2017). Carbohydrate Polymers, 161, 286-294.
又称β-D-葡糖苷醛酸酶。是作用于 D-葡糖醛酸的β -配糖体，使其葡糖醛酸苷键加水分解的酶的总称。 EC． 3． 2． 1． 31．在实验室中，为测定方便起见，在酚酞等游离后，是使用以容易比色定量的具有配质的葡糖醛酸苷为基质的方法。但因该酶对配质的特异性甚广，也能作用于以醇、类固醇、羧酸等为配质的β -D-葡糖醛酸苷。因酶来源不同而有相当大的差异。天然基质（真基质）到底如何，有时也不一定很清楚。高等植物、微生物中虽也有存在，但在动物中，该酶却几乎存在于所有的组织中，除了血浆等体液中常
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Bacteria can vary their enzymic composition markedly when grown in different conditions. In particular, specific enzymes required to utilize a given carbon or nitrogen source are usually only present when they are essential for growth
