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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
3年
- 英文名：
L-Fucose Assay Kit
- 库存：
1
- 供应商：
上海经科化学科技有限公司
- 保存条件：
常温/冷藏
- 规格：
100 assays
L-岩藻糖检测试剂盒介绍：
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Fucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 100 µg of L-fucose per assay |
| Limit of Detection: | 0.68 mg/L |
| Reaction Time (min): | ~ 10 min |
| Application examples: | L-Fucose is present as the main component in fucoidan (a marine polysaccharide), foods, pharmaceuticals and other materials (e.g. biological samples, etc.). |
| Method recognition: | Novel method |
- Very cost effective
- All reagents stable for > 2 years after preparation
- Only enzymatic kit available
- Simple format
- Rapid reaction time (~ 10 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full range of monosaccharide and disaccharide assay kits.
L-岩藻糖检测试剂盒说明书(PDF)请向客服索取！
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- 作者
- 内容
- 询问日期
文献和实验1.Measurement of available carbohydrates in cereal and cereal products, dairy products, vegetables, fruit and related food products and animal feeds: First Action 2020.07.
McCleary, B. V. & McLoughlin, C. (2021). Journal of AOAC International, qsab019.
2.Exploring the sequence-function space of microbial fucosidases.
Gascueña, A. M., Wu, H., Owen, D., Hernando, P., Monaco, S., Penner, M., Le Gall, G., Gardner, R., Ndeh, D., Urbanowicz, P., Spencer, D., Walsh, M., Angulo, J. & Juge, N. (2023). In Press.
3.Cryo-EM structures of human fucosidase FucA1 reveal insight into substrate recognition and catalysis.
Armstrong, Z., Meek, R. W., Wu, L., Blaza, J. N. & Davies, G. J. (2022). Structure, 30(10), 1443-1451.
4.Fucoidan Regulates Starch Digestion: In Vitro and Mechanistic Study.
Koh, H. S. A., Chong, J. E. L., Lu, J. & Zhou, W. (2022). Foods, 11(3), 427.
5.Discovery and characterization of a novel α-L-fucosidase from the marine-derived Flavobacterium algicola and its application in 2′-fucosyllactose production.
Zhou, W., Jiang, H., Liang, X., Qiu, Y., Wang, L. & Mao, X. (2022). Food Chemistry, 369, 130942.
问：想知道肝素如何影响a-l-岩藻糖苷酶的测定，原理是什么，哪位专家可否告知？ 答：常用抗凝剂中肝素对反应有正基质效应，分析原因可能为肝素系多聚阴离子，表面带有大量的负电荷；在弱酸性条件下（pH=4.8，AFU酶催化最适pH），反应体系中蛋白质、脂蛋白等多带正电荷，易与肝素作用形成不溶性复合物。枸橼酸盐对该反应有负基质效应，可能与其和PNP/CPNP类底物有反应，具体原因尚需进一步的证实，终点法类型试剂也存在类似的基质效应，所以不主张使用肝素\枸橼酸盐缓冲体系抗凝剂。草酸盐、NaF、EDTA
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中，最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒（BCA Protein Assay Kit）。因为BCA法蛋白定量具有很多优点，如1.实验操作十分简单方便;2.实验耗时短，快速;3.试剂灵敏度高，实验结果准确;4.所用的试剂稳定性好，而且经济实用，抗干扰能力强等。 实验原理： BCA(bicinchoninic acid)法蛋白浓度定量
