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文献和实验Natural Product Chemistry in Action: The Synthesis of Melatonin Metabolites K1 and K2
melatonin, the kynurenamines N1-acetyl-N2 -formyl-5-methoxykynurenine (K1 ) and N -acetyl-5-methoxykynurenamine (K2 ). The four key synthetic transformations involve (a) conversion of 4-methoxy aniline into the tert -butyl (4-methoxyphenyl)carbamate, (b
A Novel Method for the Production of Fully Modified K-Ras 4B
production of two structural domains—the N-terminal catalytic domain and the C-terminal peptide chemically modified with S-farnesyl-l -cysteine methyl ester. After the two domains are prepared, they are ligated together using
min in 50 µl of buffer containing 100 mM sodium phosphate (pH 7.0), 5 mM EDTA, 1 mM NADPH, 1 mM NADH, 150 µM DTT, 5 mM ß-mercaptoethanol, 20 µM acetyl-CoA or n-octanoyl-CoA or any other acyl-CoA as needed, 4% DMSO, and 8 µM of ACP pretreated with DTT
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