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文献和实验The Polymerase Chain Reaction (PCR)
• Template DNA • Reaction buffer (Tris, ammonium ions(and/or potassium ions), magnesium ions,bovine serum albumin) • Nucleotides (dNTPs) • Primers • DNA polymerase (usually Taq) PCR In Detail • Denature, anneal, extend and repeat the cycle 30 to 35
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
CCMB buffer to make chemical competent cell
but that amount was found to be 2X too much per tests on 1.23.07 --Meaganl 15:50, 25 January 2007 (EST) water to 1 liter autoclave or sterile filter Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5
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