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文献和实验Cell Migration in Confinement: A Micro-Channel-Based Assay
This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages
Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
of the players/pathways is required. For this purpose, we developed a cell migration assay based on automatic high-throughput microscopy screen. This approach allows for screening of hundreds of genes, e.g., those encoding various kinases and phosphatases
Phospholipases A2 (PLA2 ) catalyze the hydrolysis of the ester bond at the sn-2 position of phospholipids to generate the lysophospholipid and free fatty acid (1 ). Several classes of PLA2 s have been identified (2 ). They include the low
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