Biotinylated Human TGF-beta 3

Biotinylated Human TGF-beta 3 (N-Avi) (CY95)

产品说明(Description)

 

Biotinylated Recombinant Human Transforming Growth Factor Beta-3 Proprotein is produced by our Mammalian expression system and the target gene encoding Ala301­Ser412 (Tyr340Phe) is expressed with a Avi tag at the N-terminus.

Accession #: P10600

Known as:  TGFB3; ARVD; TGF-beta3

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.

 

质量控制(Quality Control)

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

Transforming growth factor beta (TGF β ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY