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文献和实验9 Assay for Serum Glycated Lipoproteins
Modified low density lipoprotein (LDL) is considered to be a risk factor for the development and progression of atherosclerosis (1 -3 ). Glycated LDL and oxidized LDL are the only modified lipoproteins that exist naturally in the human body
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Direct Serum Assay for MicroRNA in Cancer Patients
-qPCR directly in serum assay (RT-qPCR-DS) where RT is directly performed in serum without the need for RNA extraction. Results from the two protocols are analyzed and compared. RT-qPCR-DS is logistically simpler and more sensitive in assessing cmiR in breast
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