Glucose Uptake Cell-Based Assa

Study of Glucose Uptake in Adipose Cells

to its site of action. As a result, Glut4 content on the plasma membrane is increased, and glucose uptake in the cell is significantly elevated. Here, we describe two complementary techniques. The first one uses tritiated 2-deoxyglucose and is designed

Isotope-Based Quantitation of Uptake, Release, and Metabolism of Glutamate and Glucose in Cultured Astrocytes

Protocols are described for measurement in primary cultures of astrocytes of unidirectional fluxes of glutamate (influx and efflux), glutamate metabolism to glutamine or CO2 , glucose influx, glycolysis, pyruvate

Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake

(2 ,3 ). In order to measure glucose uptake, many groups have pooled embryos to increase the signal of radioactive 2-deoxyglucose transported into the blastocyst. The advantage of this technique is that single-embryo uptake can be measured using not radioactivity