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文献和实验Study of Glucose Uptake in Adipose Cells
to its site of action. As a result, Glut4 content on the plasma membrane is increased, and glucose uptake in the cell is significantly elevated. Here, we describe two complementary techniques. The first one uses tritiated 2-deoxyglucose and is designed
Protocols are described for measurement in primary cultures of astrocytes of unidirectional fluxes of glutamate (influx and efflux), glutamate metabolism to glutamine or CO2 , glucose influx, glycolysis, pyruvate
Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake
(2 ,3 ). In order to measure glucose uptake, many groups have pooled embryos to increase the signal of radioactive 2-deoxyglucose transported into the blastocyst. The advantage of this technique is that single-embryo uptake can be measured using not radioactivity
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