- 询价
- Biotium
- 美国
- 29056
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
2年
- 英文名：
Streptavidin, CF405L conjugate 29056
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
CF® dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.
- Conjugates with wide choice of CF® dye colors, plus HRP, R-PE, APC, and Alkaline Phosphatase
- Superior CF® dyes are bright, photostable, and water-soluble
- Ideal for flow cytometry, western blotting, and immunofluorescence staining
Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Streptavidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog number |
|---|---|---|---|---|
| CF®350 Streptavidin | CF®350 | 347/448 nm | 1 mg | 29031 |
| CF®405S Streptavidin | CF®405S | 404/431 nm | 1 mg | 29032 |
| CF®405M Streptavidin | CF®405M | 408/452 nm | 1 mg | 29033 |
| CF®405L Streptavidin | CF®405L | 395/545 nm | 1 mg | 29056 |
| CF®430 Streptavidin | CF®430 | 426/498 nm | 1 mg | 29065 |
| CF®440 Streptavidin | CF®440 | 440/515 nm | 1 mg | 29066 |
| CF®488A Streptavidin | CF®488A | 490/515 nm | 1 mg | 29034 |
| CF®514 Streptavidin | CF®514 | 516/548 nm | 1 mg | 29081 |
| CF®532 Streptavidin | CF®532 | 527/558 nm | 1 mg | 29030 |
| CF®543 Streptavidin | CF®543 | 541/560 nm | 1 mg | 29043 |
| CF®555 Streptavidin | CF®555 | 555/565 nm | 1 mg | 29038 |
| CF®568 Streptavidin | CF®568 | 562/583 nm | 1 mg | 29035 |
| CF®583R Streptavidin | CF®583R | 586/609 nm | 1 mg | 29086 |
| CF®594 Streptavidin | CF®594 | 593/614 nm | 1 mg | 29036 |
| CF®633 Streptavidin | CF®633 | 630/650 nm | 1 mg | 29037 |
| CF®640R Streptavidin | CF®640R | 642/662 nm | 1 mg | 29041 |
| CF®647 Streptavidin | CF®647 | 650/665 nm | 1 mg | 29039 |
| CF®660R Streptavidin | CF®660R | 663/682 nm | 1 mg | 29040 |
| CF®680R Streptavidin | CF®680R | 680/701 nm | 1 mg | 29072 |
| Alkaline Phosphatase Streptavidin (1 mg/mL) |
Alkaline Phosphatase |
N/A | 100 uL | 29071-100uL |
| 1 mL | 29071-1mL | |||
| HRP Streptavidin | HRP | N/A | 1 mg | 29049 |
| R-PE Streptavidin (0.5 mg/mL) |
R-PE | 496, 546, 565/578 nm | 200 uL | 29044-200uL |
| 1 mL | 29044-1mL | |||
| APC Streptavidin (0.5 mg/mL) |
APC | 650/660 nm | 200 uL | 29048-200uL |
| 1 mL | 29048-1mL |
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文献和实验) in coating buffer and add 50 l of the dilution to each well of an ELISA plate. Incubate overnight at 4°C. This will coat the ELISA plate with cAMP conjugate. The next day, wash the wells of the ELISA plate with HBST
). Immediately add 100μl per well. Incubate at room temperature for 2 hours. 3) Avidin-HRP Conjugate: Aspirate and wash plate 4 times. Dilute Avidin-HRP conjugate 1:2000 in diluent. Add 100μl per well. Incubate 30 min at room temperature.
ELISA Procedure for Measuring Serum Antibody Titer
. 2. Prepare buffer solutions. 3. Prepare a solution of the peptide, protein or peptide/conjugate (10 µg/mL with respect to the peptide or protein) in sodium carbonate buffer. 4. Pipette 200 µL of either peptide, peptide carrier conjugate, or sodium carbonate
