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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
2年
- 英文名:
Streptavidin, CF405M conjugate 29033
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
CF® dyes conjugates of Streptavidin are high quality biotin-binding proteins labeled with the superior CF® dyes or a selection of other labels. These conjugates are typically used as secondary reagents to detect biotinylated probes such as primary antibodies for flow cytometry, western blotting, immunofluorescence staining, and other applications.
- Conjugates with wide choice of CF® dye colors, plus HRP, R-PE, APC, and Alkaline Phosphatase
- Superior CF® dyes are bright, photostable, and water-soluble
- Ideal for flow cytometry, western blotting, and immunofluorescence staining
Blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Streptavidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog number |
|---|---|---|---|---|
| CF®350 Streptavidin | CF®350 | 347/448 nm | 1 mg | 29031 |
| CF®405S Streptavidin | CF®405S | 404/431 nm | 1 mg | 29032 |
| CF®405M Streptavidin | CF®405M | 408/452 nm | 1 mg | 29033 |
| CF®405L Streptavidin | CF®405L | 395/545 nm | 1 mg | 29056 |
| CF®430 Streptavidin | CF®430 | 426/498 nm | 1 mg | 29065 |
| CF®440 Streptavidin | CF®440 | 440/515 nm | 1 mg | 29066 |
| CF®488A Streptavidin | CF®488A | 490/515 nm | 1 mg | 29034 |
| CF®514 Streptavidin | CF®514 | 516/548 nm | 1 mg | 29081 |
| CF®532 Streptavidin | CF®532 | 527/558 nm | 1 mg | 29030 |
| CF®543 Streptavidin | CF®543 | 541/560 nm | 1 mg | 29043 |
| CF®555 Streptavidin | CF®555 | 555/565 nm | 1 mg | 29038 |
| CF®568 Streptavidin | CF®568 | 562/583 nm | 1 mg | 29035 |
| CF®583R Streptavidin | CF®583R | 586/609 nm | 1 mg | 29086 |
| CF®594 Streptavidin | CF®594 | 593/614 nm | 1 mg | 29036 |
| CF®633 Streptavidin | CF®633 | 630/650 nm | 1 mg | 29037 |
| CF®640R Streptavidin | CF®640R | 642/662 nm | 1 mg | 29041 |
| CF®647 Streptavidin | CF®647 | 650/665 nm | 1 mg | 29039 |
| CF®660R Streptavidin | CF®660R | 663/682 nm | 1 mg | 29040 |
| CF®680R Streptavidin | CF®680R | 680/701 nm | 1 mg | 29072 |
| Alkaline Phosphatase Streptavidin (1 mg/mL) |
Alkaline Phosphatase |
N/A | 100 uL | 29071-100uL |
| 1 mL | 29071-1mL | |||
| HRP Streptavidin | HRP | N/A | 1 mg | 29049 |
| R-PE Streptavidin (0.5 mg/mL) |
R-PE | 496, 546, 565/578 nm | 200 uL | 29044-200uL |
| 1 mL | 29044-1mL | |||
| APC Streptavidin (0.5 mg/mL) |
APC | 650/660 nm | 200 uL | 29048-200uL |
| 1 mL | 29048-1mL |
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文献和实验3 分线外挣扎的你,看别人如何登顶 Nature、Science
- 转运的离子通道。上篇:2012 Nature正片由楔子和四幕构成,前情交代 CF 模型猪和杀菌能力检测手段后,进入正戏。最终证明,CFTR 使 HCO3-转运减少,ASL pH 下降,而影响气道 ASL 中的抗菌物质的杀菌能力。楔子 隆重推出 CFTR 缺陷基因猪和抗菌检测金属网格突破 1建立了 CFTR 基因缺陷的 CF 模式猪突破 2金网格活体杀菌能力测量单个细菌依次固定在金网格薄片上 (Fig. 1a,b),放入气道,以此测定气道杀菌能力。通过生物素-链霉亲和素(biotin
放置。 (2)用洗涤缓冲液润洗膜。 (3)加入15ml封闭缓冲液,室温振荡30min。 (4)用1ml封闭缓冲液稀释15μl辣根过氧化物酶标记链霉亲和素(Streptavidin-HRP conjugate),加入显色盒中(不要将酶稀释液直接加在膜上),室温继续振荡45min。 (5)倒掉封闭缓冲液,用洗涤缓冲液洗涤三次,每次室温振荡10min。 (6)等体积的混合Substrate A和B。 (7)将膜放在一个平板上,用1ml底物溶液覆盖整张膜。(为了确保底物溶液在整张膜上分布均匀,可以再滴加了底物
受检物质的量直接相关,故可根据呈色的深浅进行定性或定量分析。由于酶的催化效率很高,间接地放大了免疫反应的结果,使测定方法达到很高的敏感度。 二、ELISA的类型 ELISA可用于测定抗原,也可用于测定抗体。在这种测定方法中有三个必要的试剂:(1)固相的抗原或抗体,即"免疫吸附剂"(immunosorbent);(2)酶标记的抗原或抗体,称为"结合物"(conjugate);(3)酶反应的底物。根据试剂的来源和标本的情况以及检测的具体条件,可设计出各种不同类型的检测方法。用于临床检验的ELISA











