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NucSpot® Live 488 Cell Nuclear

Stains,10ul 40081-T
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  • 询价
  • Biotium已认证
  • 美国
  • 40081-T
  • 2026年01月24日
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    2金牌会员
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      4°C避光

    • 保质期

      12个月

    • 英文名

      NucSpot® Live 488 Cell Nuclear Stains,10ul 40081-T

    • 库存

      99

    • 供应商

      Biotium

    • CAS号

      1

    NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.

    Features

    • No-wash, nuclear-specific DNA dyes
    • Low-toxicity for real-time live cell imaging
    • Fix before or after labeling
    • NucSpot® Live 650 compatible with SIM, STED, or STORM
    • Choose green or far-red fluorescence

    Kit Components

    • NucSpot® Live Dye, 1000X in DMSO
    • Verapamil, 100 mM in DMSO

    Spectral Properties

    • NucSpot® Live 488 Ex/Em: 500/515 nm
    • NucSpot® Live 650 Ex/Em: 650/675 nm

    NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 4). The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of the efflux pump inhibitor verapamil for optional use, which may increase probe retention and live cell staining in some cell types. Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

    NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

    Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.

    Cy Dye is a registered trademark of GE Healthcare.

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    图标文献和实验
    相关实验
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    • LIVE/DEAD® Violet Viability/Vitality Kit

      protocol has been optimized using Jurkat cells (human T-cell leukemia line) at a concentration of 1 × 106 cells/mL. Use of other cell types or other cell concentrations may require optimization of staining. If another staining reaction is to be performed

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      of living cells using a dead cell discriminating stains such as SYTOX® Green, SYTOX® Red or SYTOX® AADvanced™ dead cell stains or LIVE/DEAD® Fixable Dead cell stains such as Green, Red, Far Red, or Near-IR kits Compensation may be required for Alexa

    图标技术资料

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    资料下载:

    PI-40081-1.pdf 附 (下载 0 次)

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