- $280
- LSM BIO
- 进口
- PVT39627
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20℃
- 保质期：
3个月
- 英文名：
pEnCMV-3×Myc-MIB2(1-392aa)-SV40-Neo
- 库存：
50
- 供应商：
LSM BIO
- 规格：
2ug
pEnCMV-3×Myc-MIB2(1-392aa)-SV40-Neo
PVT39627 2ug
pEnCMV-3×Myc-MIB2(1-392aa)-SV40-Neo Description
Alias:NM_080875.5;ZZANK1; ZZZ5Clone strain:DH5a
Culture conditions:37centigrade
Host：Human
Gene type:ORF
Prokaryotic resistance：Amp
Use：Protein expression
Note:
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20ul ddH2O in to the tube of plasmid.
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文献和实验AA Metabolite Quantitation of Media Post-AA Labeling
TLC plate for TLC system #1, use 20µl to spot a linear K TLC plate for TLC system #2. TLC : A) Allow TLC tanks to equilibrate 1-2 hrs with solvent prior to running. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate
AA Metabolite Quantitation of Cell Pellets Post-AA Labeling
. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate/2,2,4-trimethylpentane/acetic acid/ H2O at a ratio of 90:50:20:100 SYSTEM #2 --> Separates DAG & AA. benzene/diethyl ether/ethanol/NH3 at a ratio of 100:80:4:0.2 B) Following TLC remove plates
AA & Metabolite Quantitation of Media Post-AA
AA & Metabolite Quantitation of Media Post-AA Labeling Contributor: Suprya Jayadev Date: November 4, 1994 1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count
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