- $280
- LSM BIO
- 进口
- PVT38293
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20℃
- 保质期：
3个月
- 英文名：
pEnCMV-B602L(virus)-3×FLAG-SV40-Neo
- 库存：
50
- 供应商：
LSM BIO
- 规格：
2ug
pEnCMV-B602L(virus)-3×FLAG-SV40-Neo
PVT38293 2ug
pEnCMV-B602L(virus)-3×FLAG-SV40-Neo Description
Alias:Codon optimizationClone strain:DH5a
Culture conditions:37centigrade
Host：virus
Gene type:ORF
Prokaryotic resistance：Amp
Use：Protein expression
Note:
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20ul ddH2O in to the tube of plasmid.
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文献和实验Virus Infection of Fibroblasts
containing 10% calf serum (prepare dilutions just before use): a. 1:10 (150 μl virus stock + 1,350 μl media) b. 1:100 (150 μl of 1:10 virus stock + 1,350 μl media) c. 1:1,000 (150 μl of 1:100 virus stock + 1,350 μl media) d. 1:10,000 (150 μl
. (virus can be stored at 4C for one week, otherwise, aliquot virus and store at -80C for further use) B. Infection: 9. Seed cells that need to be infected in a 12-well or 6-well plate. The best confluence is 40-70%, depending on the different
and thereby prevents infection of a susceptible cell. The mechanisms of virus neutralization are not fully understood. Neutralization is not simply a matter of coating the virion with antibody, nor indeed of blocking attachment to the host cell. In fact, neutralized
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