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2
- 规格：
1 × 120 mL
| Parameter | HiLoad 16/600 Superdex 200 pg |
|---|---|
| Bed dimensions | 16 × 600 mm |
| Bed height | 600 mm |
| Includes | Dismantling tool, support screen, net ring (10 μm), O-ring, stop plugs, and instructions. |
| Bed volume | 120 |
| Column i.d. | 16 mm |
| Flow rate1 | 1.6 ml/min |
| Flow velocity | 10 cm/h - 50 cm/ |
| Order Information | HiLoad columns are prepacked XK columns. |
| Sample volume | < 5 ml |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 1 × 120 mL |
HiLoad Superdex 200 pg prepacked columns are for high-resolution size exclusion chromatography of mAbs and other proteins from samples up to 5 mL or 13 mL.
- Prepacked format – for convenience and reproducibility; can be used with standalone pump or chromatography system.
- High resolution – for high purity.
- Scalable – separations can be scaled up using Superdex 200 prep grade resin.
- ÄKTA system compatible – Fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems.
Gel filtration column selectivity
This column is packed with Superdex 200 prep grade (pg) resin. This SEC resin combines excellent mechanical strength with high hydrophilicity, allowing high flow rates and minimal nonspecific interactions. Steep selectivity curves give excellent resolving power for proteins and other biomolecules in the molecular weight range: Mr ~ 10 000 to 600 000.
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文献和实验Size‐Exclusion Chromatography with On‐Line Light Scattering
the use of size?exclusion chromatography with on?line light scattering, UV absorbance, and refractive index detectors (SEC?LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight
Purification of Antiserum or Ascites by Protein A/G Chromatography
Agarose Affinity ColumnTypically, columns containing 5 ml or 10 ml of packed protein A/G Agarose are prepared. The size of the column is determined by the binding capacity of protein A/G and the amount of antiserum that must be processed. Protein
. Roeder, eds.) pp. 1‐94. Academic Press, New York. Becker, G.W. 1988. Frontal boundary analysis in size exclusion chromatography of self‐associating proteins. In Aqueous Size
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