
Qiagen QIAquick PCR Purificati
on Kit 28104- ¥1600
- QIAGEN
- 28104
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
纯化多至10 μg PCR产物,100 bp–10 kb
- 即用型DNA回收率高达95%
- 3个简单步骤即可回收多达10 kb DNA片段
- 含有凝胶上样染料,方便样本分析
QIAquick PCR Purification Kit包含离心柱、缓冲液和收集管,利用硅胶模技术纯化100 bp以上的PCR产物。 通过简单快速的结合、洗涤、洗脱步骤,使用30–50 μl洗脱体积即可纯化多达10 kb的DNA片段。内置的pH指示剂可方便测定DNA与离心柱结合的最佳pH值,整个过程可在QIAcube全自动核酸纯化仪上自动化运行。
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
PCR product purification-Molecular Biology
I have tried to purify my PCR products by using 3 different approaches:1) QIAQuick PCR purification kit: but when I run the gel to see its efficacy, there was simply nothing!! it cleaned everything including my band!!2)Zymogen's Cleaning
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