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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
纺锤体和着丝粒相关蛋白3抗体
- 抗体英文名:
SKA3
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF7848R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
SKA3纺锤体和着丝粒相关蛋白3抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

SKA3纺锤体和着丝粒相关蛋白3抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:SKA3纺锤体和着丝粒相关蛋白3抗体
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文献和实验,否则会杀死细胞,0.2%左右的浓度诱导效果最好。 6、 有丝分裂各个时期总结为:“间期复制,前期三体,中期上道,后期加倍,末期反前”。 “间期 复制 ”是指,间期的染色体要进行复制,包括DNA的复制和相关蛋白质的合成。但此时的染色体是丝状的,又叫染色质。间期也可叫染色单体产生的时期。染色体复制后的二条染色体,因为共一个着丝点,只能叫包含有二条染色单体的一条染色体,间期的细胞核明显,有核膜和核仁。 “前期 三体 ”是指,出现染色体,出现纺锤体,核膜核仁解体。出现染色
的时期。 Cell hybrid (细胞杂交):包含来自不同种属亲本细胞染色体的体细胞(如人-鼠融合细胞杂交),通过融合细胞形成融合的异型核而产生。 Centrioles (中心粒):在减数分裂期聚集在中轴附近、由微管组成的小空圆柱体,位于着丝粒上。 Centromere (着丝粒):染色体聚集区域,包含减数分裂或有丝分裂纺锤体结合位点。 Centrosomes (中心体):减数分裂细胞微管组织的区域。在动物细胞中,每一个中心体包括一对由微管附接的、高密度不定型区域围绕的中心粒构成
群落。 Micrococcal nuclease (微球菌核酸酶):切割DNA 的内切核酸酶,在染色质核小体之间的 DNA 更容易被切开。 Microsomes (微粒体):与核糖体结合的碎片状内质网。 Microtubules (微管):由微管蛋白质二聚体组成的纤维。中期的微管被重新组织成有丝分裂 中的纺锤体纤维,负责染色体的移动。 Microtubule associated proteins (微管相关蛋白,MAPs):与微管相关的蛋白质,影响微管稳 定性和组织
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