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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
中心粒蛋白Nudel抗体
- 抗体英文名:
NUDEL
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF4141R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
NUDEL中心粒蛋白Nudel抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

NUDEL中心粒蛋白Nudel抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:NUDEL中心粒蛋白Nudel抗体
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文献和实验模型和人群样本,发现由肝脏分泌的Gpnmb 蛋白可作用于脂肪组织,促进脂质合成,参与肥胖及胰岛素抵抗的病程。而运用抗体中和等方法降低血液中的 Gpnmb 可以有效抑制脂肪组织合成脂质的能力,提高机体能量消耗,减轻肥胖并改善胰岛素抵抗。研究揭示了在代谢性疾病等情况下肝脏和脂肪组织的一种通讯机制,也证明靶向 Gpnmb 可治疗肥胖和糖尿病等。根据世界卫生组织统计,2016 年,18 岁及以上的成年人中逾 19 亿人超重,其中超过 6.5 亿人肥胖。肥胖是糖尿病、脂肪肝和心脑血管疾病的重要危险因素,可受遗传
发表于“HEPATOLOGY”(《肝病学》)杂志。 文章探讨了趋化因子CXCL5在HCC中的作用机制,首先在细胞水平对CXCL5和中心粒细胞浸润与HCC之间的关系做了大量的推测和实验验证,并且发现了CXCL5通过两个主要的信号通路PI3K-Akt 和ERK1/2参与到HCC的过程中。特别是在体外检测CXCL5对中性粒细胞的引诱实验中,用到了磷酸化抗体芯片这种高通量的蛋白检测分析平台。磷酸化的抗体芯片(课题相关蛋白芯片服务由上海伯豪生物技术有限公司提供)不仅能够检测出蛋白水平的变化,同时还能够检测蛋白磷酸化修饰水平的变化
体内甲醛交联及染色质免疫沉淀--研究DNA和蛋白质作用的新方法
分析释放出的组蛋白。上个世纪九十年代初,免疫沉淀用于分离固定的复合物,vasrhavshy等〔`〕使用组蛋白抗体研究DNA与转录的关系,从而形成了甲醛交联及染色质免疫沉淀的方法。上世纪九十年代,抗体滴度和特异性大大提高,比如针对组蛋白转录后修饰的乙酞化、磷酸化的特异性抗体相继出现,为染色质修饰和基因表达活性等的研究大开方便之门。在染色质结构和动力学中的应用甲醛交联及染色质免疫沉淀方法已成功用于研究染色质结构和染色质动力学等不同的领域中。研究对象涉及酵母、原虫、果蝇仁、人体细胞以及古细菌毛等多种生物
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