相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
胆盐激活脂肪酶抗体
- 抗体英文名:
Bile salt-activated lipase
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF5042R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Bilesalt-activatedlipase胆盐激活脂肪酶抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Bilesalt-activatedlipase胆盐激活脂肪酶抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:Bilesalt-activatedlipase胆盐激活脂肪酶抗体
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验到胆汁中。 胆固醇 人肝胆汁中含0.6~0.7克/升的非酯化胆固醇,1~5克/升卵磷脂和3克/升的中性脂肪。胆固醇由乙酰辅酶A合成,其生物合成部位在肝和肠,在细胞的微粒体中合成。人每天合成胆固醇约1~1.3克,其中0.4~0.6克在肝内转变成胆汁酸。 磷脂 在肝细胞中合成,部位尚不清楚。人每天约有300~600毫克磷脂分泌入胆汁。 胆汁的作用 主要是胆盐和胆酸作用。能激活胰脂肪酶,使分解脂肪的速度增加。胆盐、胆固醇、卵磷酯可作为乳化剂,降低脂肪的表面
酶( pancreatolipase)可将脂肪分解为甘油和脂肪酸,含三种脂肪水解酶:一为甘油一酸脂的水解酶,可水解在水、油介面上的不溶性甘油酯,需胆盐及相应的表面活化剂一起作用,最适 pH7~ 9;第二种水解二级醇或其他醇的酯,需与胆盐一起作用, pH8;第三种水解水溶性酯的酶。还有磷脂酶 A,能将卵磷脂水解成溶血卵磷脂。胰蛋白酶( trypsin)与糜蛋白酶( chymotrypsin)由胰腺腺泡细胞分泌,以无活性酶原形式存在于胰液中。胰蛋白酶原可被肠液中的肠致活酶激活成有活性的胰蛋白酶。酸及胰蛋白酶本身亦可激活胰蛋白
形成一种高亲和度的复合物,牢固地附着在脂肪颗粒表面,防止胆盐把脂肪酶从脂肪表面置换下来。因此,辅脂酶的作用可比喻为附着在甘油三酯表面的“锚”。 胰液中还含有一定量的胆固醇和磷脂酶A 2 ,它们分别水解胆固醇酯和卵磷脂。 3. 胰蛋白酶和糜蛋白酶 这两种酶者是以不具有活性的酶原形式存在于胰液中的。肠液中的肠致活酶可以激活蛋白酶原,使之变为具有活性的胰蛋白酶。此外,酸、胰蛋白酶本身,以及组织液也能使胰蛋白酶原活化。糜蛋白酶原是在胰蛋白酶作用下转
技术资料暂无技术资料 索取技术资料









