相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
呼吸道合胞病毒抗体
- 抗体英文名:
RSV Major surface glycoprotein G
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF1264R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
RSVMajorsurfaceglycoproteinG呼吸道合胞病毒抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

RSVMajorsurfaceglycoproteinG呼吸道合胞病毒抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:RSVMajorsurfaceglycoproteinG呼吸道合胞病毒抗体
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人抗呼吸道合胞病毒抗体 (RSV) 酶联免疫分析(ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于检测人血清,血浆及相关液体样本中抗呼吸道合胞病毒抗体(RSV) 水平,感染人的血液学诊断。 实验原理: 本试剂盒采用双抗体夹心酶联免疫法( ELISA )测定标本中人抗呼吸道合胞病毒抗体 (RSV) 。用纯化的 抗体 包被微孔板,制成固相抗体,可与样品中抗呼吸道合胞病毒抗体 (RSV
Nature:施一 / 高福团队揭示埃博拉病毒聚合酶工作的分子机制
蛋白(VP24)结合,并被基质蛋白(VP40)环绕,进一步与病毒表面刺突糖蛋白(GP)形成完整病毒颗粒。病毒表面 GP 蛋白在细胞入侵和免疫逃逸方面发挥着重要作用。目前已上市的两款针对扎伊尔型埃博拉病毒的抗体药物,其作用靶点就是埃博拉病毒表面 GP 蛋白。但是抗体药物在制造成本、存储和使用便利性上面具有局限性,而且病毒 GP 蛋白在宿主免疫压力下容易发生免疫逃逸,造成抗体药物效果变差甚至无效。此外由于抗体药物的特异性,无法用于治疗其他类型埃博拉病毒以及马尔堡病毒等丝状病毒。因此,开发有效安全的广谱性抗病
结果。实验中,应尽量利用经微生物控制的动物和纯种动物。1. 黄热病毒:灵长类动物都易感,最为敏感的动物是恒河猴,另外可用中国猴(Macacus Sinicus)及断尾猴(M.Speciosus Thibetanus)。小鼠对黄热病毒也敏感,在没有经济实力,使用灵长类时,可用小鼠。令人感兴趣的是:纯血清接种的小白鼠尚能存活,而以稀释血清接种的小白鼠却于较短时间死亡。这一奇特现象的理由,可能是由于病人体内血清抗体早期出现之故。应用纯血清时,这些抗体中和了病毒的作用,而当应用稀释血清时,抗体浓度不足,但病毒
技术资料暂无技术资料 索取技术资料









