相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
信号感应增殖相关蛋白1抗体
- 抗体英文名:
SPA-1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF1602R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
SPA-1信号感应增殖相关蛋白1抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

SPA-1信号感应增殖相关蛋白1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:SPA-1信号感应增殖相关蛋白1抗体
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验网络平衡阈值的跨越及对增殖信号的感应 早在20世纪初,PaulEhrlich就在有关抗体产生的侧链理论中提出,抗体形成细胞可表达结构各异的多种受体,一旦抗原出现,结构与之互补的受体分子通过与该抗原的结合,可使相应细胞产生并大量分泌同样类型的抗体分子。在Ehrlich绘就的一张示意图中,生动地展示了抗体生成细胞如何“知道”其受体已与抗原结合,并迅速做出应答。该示意图的下半部可见,参与结合的受体分子越多,感应细胞所分泌的抗体分子就越多(右下方的细胞看起
重磅综述:年发文量近 10000,自噬领域第 4 版研究指南发布
过程中的分子机制;研究多细胞生物发育过程中,自噬如何感应各种信号刺激进而维持机体稳态平衡;揭示自噬作用在多细胞生物发育过程中的生理学功能,尤其是自噬异常在神经退行性疾病发生发展中的作用。2、钟清上海交通大学基础医学院研究员,团队致力于研究关键自噬调控因子在自噬体形成,底物选择和自噬体 - 溶酶体融合中生化功能解析和重建;并进一步探讨自噬在脂质代谢中的功能。他的实验室还涉及了关于氧化应激坏死的新机制。3、俞立 清华大学生命科学学院教授,研究方向综合细胞生物学,遗传学,生物化学,分子生物学等研究
三句话读懂一篇 CNS:代糖也有害,增加焦虑风险 | 每天运动仅 3 分钟,死亡风险降低 40%
相关蛋白 LKB1-SIK3 激酶以及组蛋白脱乙酰化酶 HDAC4 和 HDAC5 对于睡眠持续时间的调节,HDAC4 和 HDAC5 的磷酸化与睡眠需求增加有关,证明了体细胞遗传操纵对于小鼠睡眠研究的重要性。 图 4:来源 Nature 5. STTT:发现具有广谱抗癌活性的强效 USP25/28 抑制剂 每年因癌症失去生命的人不计其数,因此如何攻克癌症成为了科学家们最关注的问题。 2022 年 12 月 8 日,浙江大学张普民、梁廷波及中国科学院上海药物研究所谭昌恒共同
技术资料暂无技术资料 索取技术资料









