- ￥1880
- 晶风生物
- TF11194R
- 中国
- 2025年07月11日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
少突胶质细胞转录因子2抗体
- 抗体英文名：
OLIG2
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF11194R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
OLIG2少突胶质细胞转录因子2抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
OLIG2少突胶质细胞转录因子2抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验，为了实现基因靶向转移，可以构建一种可溶性分子复合物载体系统，它包含两个功能区：DNA结合区，常用L―多聚赖氨酸、脂质体等，它们通过静电方式结合DNA或将DNA包裹；配体或抗体区，它们通过识别肿瘤细胞表面受体或抗原，选择性地内化进入肿瘤细胞。Uherek等将酵母转录因子Gal4的DNA结合结构域与白喉毒素转膜结构域、抗ErbB2抗体片段依次连接，构建了一种新的融合蛋白载体，它可与质粒形成复合物，用L―多聚赖氨酸处理后，可特异性地将外源基因导人ErbB2阳性的肿瘤细胞中。
种途径影响免疫系统，且此效应存在较大的种属差异，如小鼠、大鼠、仓鼠和兔较为敏感，而豚鼠、猴和人相对不敏感，主要差异表现为后者的淋巴细胞不易被GC作用反致溶。 （1）GC影响胚胎期免疫系统的发育：如小鼠胚胎胸腺在GC作用下，其淋巴细胞表达Thy1.2抗原增加，而高浓度的GC可杀伤小淋巴细胞。胸腺的上皮细胞对GC尤为敏感。GC引起胸腺萎缩的机制涉及细胞程序性死亡或凋零（apoptosis），此作用需要核酸内切酶的参与，在Ca 2+ 及Mg 2+
crinology. First published April 3, 2003 as doi:10.1210/ me. 2002- 0410 Title：Peroxisome Proliferator-activated Receptor γ Inhibits Expression of Minichromosome Maintenance Proteins in Vascular Smooth Muscle Cells 过氧化物酶体增殖子激活受体γ（PPARγ）抑制血管平滑肌细胞内微型染色体维系蛋白
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