- ￥1880
- 晶风生物
- TF0577R
- 中国
- 2025年07月16日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
白介素2受体a链/IL-2RA抗体
- 抗体英文名：
IL2RA/CD25
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF0577R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
IL2RA/CD25白介素2受体a链/IL-2RA抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IL2RA/CD25白介素2受体a链/IL-2RA抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购：IL2RA/CD25白介素2受体a链/IL-2RA抗体
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文献和实验蛋白。正是由于Cdk2和周期素E的活化，T细胞开始分裂并进入细胞周期。据观察，激活的T细胞ld可以分裂2-3次，产生大量子代细胞。 显然，T细胞没有进入激活状态，就不会有各种基因的表达，包括IL2和IL2Ra编码基因，因而也无法启动由细胞因子IL2受体介导的信号转导。换言之，细胞因子IL-2一般不会作用于未致敏和未被激活的CD4 T细胞，因为这些细胞表面不能有效地构成由α、β、γ三链组成的高亲和力ID2受体。 另外需要指出的是，IL2R。又称CD25
在初始T细胞、活化T细胞、效应T细胞和记忆T细胞表面的表达格局。揭示了两个重要现象：①抗原刺激下发生的免疫应答强度有一个常态分布曲线，与该曲线相一致的是II．-2Ra链(CD25)表达水平的涨落。本章前面提到，CD25在T细胞得到双重信号后出现，是T细胞激活的标志。从图右侧可以看出，T细胞随着应答消失而发展成记忆细胞，该标志的表达也进人低潮。②与此相反，IL-7Ra、IL-15Ra和IL-15Rp(后者同为IL-2R吕)的表达格局则完全不同。II；7Ra出现从高到低，再从低升高的表达格局，在记忆
间接免疫荧光法检测样品中mIL-2R α + 阳性细胞 白介素2受体（interleukin 2 receptor， IL-2R）是能与白介素2（IL-2）特异性结合的细胞受体。IL-2R由α、β、γ链组成。IL-2R存在形成包括膜结合型IL-2R（membrane-bound IL-2R，mIL-2R）与可溶性IL-2R（solubble IL-2R，sIL-2R）。sIL-2R是由于某种因素（如位点特异性蛋白酶裂解作用）使mIL-2R α链（P55
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