Cloning Neuropeptide Tyrosine cDNA
The nervous system is composed of a diverse population of cells selectively expressing different genes which ultimately determine the type of neurotransmitters and neuropeptides produced and released. Neuropeptide tyrosine (NPY) is one of the most abundant and widely expressed p ...
来源:互联网 650 阅读
Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1,2). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N-acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used in attempts to assess the role of calpain in degra ...
来源:互联网 650 阅读
Ion Channel Pore Sizing in Patch-Clamp Experiments
The physical dimensions of an ion channel pore can be estimated in patch-clamp experiments using various charged and noncharged molecular probes. Measuring the permeability to organic ions of different size and shape yields an estimate of the size of the narrowest part of the selectivity fi ...
来源:互联网 650 阅读
GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing
GABAA receptor (GABAAR) constitutes the main inhibitory receptor of the central nervous system. Due to the wide distribution and activity of its main neurotransmitter agonist, the γ-aminobutyric acid (GABA), its pharmacology has been thoroughly studied, given rise to the developme ...
来源:互联网 649 阅读