Patch-Clamp Techniques Applied To Brain Slices
Brain slices have become an integral part of synaptic and cellular physiology since the pioneering studies by Henry McIlwain (Li and McIlwain, 1957; Yamamoto and McIlwain, 1966). The hippocampal slice preparation was first brought to the United States from Per Anderson’s lab-initially by . ...
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Southwestern Blots for Detection of a DNA Binding Protein Recognizing the α1B-Adrenergic Receptor Gene Promoter
The α1B-adrenergic receptor (α1B-AR) is a G-protein-coupled receptor that plays a key role in the sympathetic regulation of a variety of physiological processes, such as cardiac and smooth muscle contractility, contraction of the spleen, liver glycogenolysis, melatonin secretion . ...
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Ion Channel Pore Sizing in Patch-Clamp Experiments
The physical dimensions of an ion channel pore can be estimated in patch-clamp experiments using various charged and noncharged molecular probes. Measuring the permeability to organic ions of different size and shape yields an estimate of the size of the narrowest part of the selectivity fi ...
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GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing
GABAA receptor (GABAAR) constitutes the main inhibitory receptor of the central nervous system. Due to the wide distribution and activity of its main neurotransmitter agonist, the γ-aminobutyric acid (GABA), its pharmacology has been thoroughly studied, given rise to the developme ...
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