该产品被引用文献 文献:IL-6 and IL-8 secreted by tumour cells impair the function of NK cells via the STAT3 pathway in oesophageal squamous cell carcinoma https://pubmed.ncbi.nlm.nih.gov/31324197/ 三一造血(STEMERY)是一家由留学归国学者创办的致力血液细胞分离;培养及应用的国家高新技术企业。公司目前围绕血液细胞已经形成了一条特色产品服务于基础研究,药物开发以及细胞治疗工业等客户。1FLOSEP血液细胞分离技术2血液原代细胞库;3血液细胞培养产品; 4血液细胞改造防治肿瘤技术;5血液细胞体外大规模扩增(人工血液)技术。我们的目标是从细胞免疫学角度解决肿瘤,自身免疫性疾病以及血荒等难题。 公司自创办以来,先后获得过“人社部优秀留学人员创业项目”“福建省留学人员重点支持项目”“福州市优秀留学人员支持项目”“福州市五四青年奖章”“厦门市双百人才”“福建省高层人才”“中国创新创业大赛福建赛区二等奖”等荣誉称号。我们从一小小的梦想和行动起步,将脚踏实地把“小细胞助力大健康”的梦想变成现实,矢志不渝。
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IL-6 and IL-8 secreted by tumour cells
impair the function of NK cells via the
STAT3 pathway in oesophageal squamous
cell carcinoma
In vitro co-culture of primary oesophageal squamous
carcinoma and NK cells
Peripheral blood NK cells were purified by a Human NK
Cell Enrichment Set-DM kit (BD) and then expanded
with an NK Cell Robust Expansion kit (Stemery) according to the manufacturer’s instructions. The purity of NK
cells was > 90%, and the number of cells was increased
500–1000-fold. The primary oesophageal squamous carcinoma cell supernatant was collected and cultured with
NK cells for 72 h. According to each group, anti- IL-8 or
anti- IL-6 was added (Additional file 1) or not. Finally,
the NK cell surface receptor expression levels, proliferation and cytokine expression levels were detected by
flow cytometry