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抗人egfr单克隆抗体
- 参考价格： ¥3575.0
- 品 牌： abgent
- 货 号： AJ1252j
- 适应物种： 人
- 应用范围： 免疫印迹,免疫组化,免疫荧光
- 克 隆 性： 单克隆
- 修 饰： pY1068
- 抗体类型： 一抗
产品概览
| 背景： | Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Additionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4).REFERENCES1. Hunter, T. The epidermal growth factor receptor gene and its product. Nature 311: 4142. Reynolds, F.H. Jr., et al. Human transforming growth factors induces tyrosine phosphorylation of EGF receptors. Nature 292: 2593. Helin, K., et al. The biological activity of the human epidermal growth factor receptor is positively regulated by its C-terminal tyrosines. Oncogene 6: 8254. Batzer, A.G., et al. Hierarchy of binding site for Grb2 and Shc on the epidermal growth factor receptor. Mol. Cell. Biol. 14: 5192 |
| 其他名字： | EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1 |
| 克隆名： | Y38 |
靶点信息
| 基因id： | 1956 |
| 蛋白名： | EGFR |
| 胞内定位： | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) |
| 同义词： | ERBB, ERBB1, HER1 |
应用与保存
| 稀释浓度： | WB~~1:20000IHC~~1:50 |
| 注意事项： | EGFR Antibody Phospho (pY1068) is for research use only and not for use in diagnostic or therapeutic procedures. |
其他
| 参考文献： | 1. Hunter, T. The epidermal growth factor receptor gene and its product. Nature 311: 4142. Reynolds, F.H. Jr., et al. Human transforming growth factors induces tyrosine phosphorylation of EGF receptors. Nature 292: 2593. Helin, K., et al. The biological activity of the human epidermal growth factor receptor is positively regulated by its C-terminal tyrosines. Oncogene 6: 8254. Batzer, A.G., et al. Hierarchy of binding site for Grb2 and Shc on the epidermal growth factor receptor. Mol. Cell. Biol. 14: 5192 |
| 保存条件： | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 标记物： | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 主要序列号： | P00533 |
| 分子量： | 134277 Da |
| 功能： | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. |
| 规格： | 100µl | 价格： | ¥3575.00 |
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