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msh2单克隆抗体
- 参考价格： ¥3025.0
- 品 牌： abgent
- 货 号： AO1025a
- 适应物种： 人,猴
- 应用范围： 免疫印迹,免疫组化,免疫荧光
- 克 隆 性： 单克隆
- 亚 型： Mouse IgG1
- 抗体类型： 一抗
产品概览
| 背景： | MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.REFERENCES1. Papadopoulos, N. 1994. Science 263: 1625-1629.2. Palombo, F. 1994. Nature 367:417-418. |
| 其他名字： | FCC1; COCA1; HNPCC; LCFS2 |
| 克隆名： | 1B3=3A2B8C |
靶点信息
| 基因id： | 4436 |
| 蛋白名： | MSH2 |
| 胞内定位： | Nucleus (Potential). |
应用与保存
| 稀释浓度： | WB~~1:500~2,000IHC~~1:200~1,000IF~~1:200~1,000 |
| 注意事项： | MSH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
其他
| 参考文献： | 1. Papadopoulos, N. 1994. Science 263: 1625-1629.2. Palombo, F. 1994. Nature 367:417-418. |
| 保存条件： | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 标记物： | Purified recombinant fragment of human MSH2 expressed in E. Coli. |
| 主要序列号： | P43246 |
| 其他序列号： | NP_000242.1 |
| 分子量： | 104743 Da |
| 功能： | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
| 规格： | 0.1ml | 价格： | ¥3025.00 |
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