Recombinant Human EGF
产品说明(Description) Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed. Accession #: P01133 Known as: Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
制剂(Formulation) Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
质量控制(Quality Control) Purity: Greater than 95% as determined by reducing SDS-PAGE. Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Bioactivity:Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml.
复溶(Reconstitution) Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
保存(Storage) Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
背景(Background) Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
电泳(SDS-PAGE)
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FOR RESEARCH USE ONLY |